anti-CD18 mouse monoclonal, 68-5A5, purified

Cat. No. 691564

Available, delivery time 1-7 days

€340.00*

Key Features
  • Mouse monoclonal
  • Suitable for FACS, ICC/IF, IHC and WB
  • Reacts with human
  • Isotype: IgG2a kappa
Product description
Quantity 1 ml (100 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG2a kappa
Conjugate Unconjugated
Application FACS, ICC/IF, IHC, WB
Purification Affinity chromatography
Reactivity Human
Storage 2-8°C
Intended use Research use only
Clone 68-5A5
Immunogen Stimulated human leucocytes
Concentration 100 µg/ml
Formulation PBS with 0.02% sodium azide
UniprotID P05107 (Human)
Synonym Integrin beta-2, Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta, Complement receptor C3 subunit beta, CD antigen CD18, ITGB2, CD18, MFI7
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:100-1:200 (0.5-1.0 µg/ml)
Immunohistochemistry (IHC) - frozen 1:50-1:100 (1-2 µg/ml)
Flow Cytometry (FACS) 0.5-1.0 µg/million cells in 0.1 ml
Western Blot (WB) 1:50-1:100 (1-2 µg/ml)
Background

It recognizes a transmembrane glycoprotein of 95 kDa, identified as CD18 or integrin-2 (Workshop III). It complexes non-covalently with either L, M, or X integrin (CD11a, b, or c) to form the heterodimers. LFA-1, MAC-1, and p150,95, respectively. LFA-1 is the receptor for three members of the Ig supergene family of proteins, ICAM-1 (CD54, ICCAM-2 (CD102), and Mac-1 and p150,95 bind to ICAM-1, fibrinogen, and IC3b. ICAM-3 (CD50). CD18/CD11 heterodimeric molecules are involved with cell/cell and cell/extracellular adhesion in immune and inflammatory responses. This Mab blocks these cellular interactions. 68-5A5 was clustered at the IIIrd International Workshop on human leucocyte differentiation antigens.

Positive control: human PBL and tonsil.

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