Diagnostic marker

anti-CD20 mouse monoclonal, 93-1B3, purified

Cat. No. 691566

Available, delivery time 1-7 days

€340.00*

Key Features
  • Mouse monoclonal
  • Suitable for FACS and IHC
  • Reacts with human
  • Isotype: IgG1 kappa
Product description
Quantity 1 ml (100 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG1 kappa
Conjugate Unconjugated
Application FACS, IHC
Purification Affinity chromatography
Reactivity Human
Storage 2-8°C
Intended use Research use only
Clone 93-1B3
Immunogen Stimulated human leucocytes
Concentration 100 µg/ml
Formulation PBS with 0.02% sodium azide
UniprotID P11836 (Human)
Synonym B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, CD antigen CD20, MS4A1, CD20
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:50-1:100 (1-2 µg/ml)
Flow Cytometry (FACS) 0.5-1.0 µg/million cells in 0.1 ml
Background

93-1B3 binds with CD20, which is a 30/33 kDa non-glycosylated transmembrane phosphoprotein with three extensive hydrophobic regions. CD20 is involved in regulation of B-cell activation. It is expressed on the surface of all B-cells beginning at the pro-B phase (CD45R+, CD117+) and progressively increasing in concentration until maturity. Plasma cells are negative. CD20 is retained on many B-cell malignancies. CD20 positive cells are also sometimes found in cases of Hodgkin's disease, myeloma, and thymoma. 93-1B3 has been clustered at the IIIrd and Vth HLDA Workshops.

Positive control: Daudi, Raji, U266, human lymphocytes, lymph nodes and tonsils.

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