anti-CD25/IL2RA mouse monoclonal, 143-13, purified

Cat. No. 691570

Available, delivery time 1-7 days


Key Features
  • Mouse monoclonal
  • Suitable for ELISA, FACS, ICC/IF, IHC and WB
  • Reacts with human
  • Isotype: IgG1 kappa
Product description
Quantity 1 ml (100 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG1 kappa
Conjugate Unconjugated
Application ELISA, FACS, ICC/IF, IHC, WB
Purification Affinity chromatography
Reactivity Human
Storage 2-8°C
Intended use Research use only
Clone 143-13
Immunogen Stimulated human leucocytes
Concentration 100 µg/ml
Formulation PBS with 0.02% sodium azide
UniprotID P01589 (Human)
Synonym Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55, CD antigen CD25, IL2RA
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:50-1:100 (1-2 µg/ml)
Immunohistochemistry (IHC) - frozen 1:50-1:100 (1-2 µg/ml)
ELISA Assay dependent
Flow Cytometry (FACS) 1-2 µg/million cells
Western Blot (WB) 1:50-1:100 (1-2 µg/ml)

143-13 reacts with CD25 (55 kDa) which binds to IL-2 with low affinity, unless it associates as alpha chain with CD122 and the common gamma chain (CD132) to form the high-affinity IL-2 receptor complex. The CD25 molecule reveals three epitope regions: A, B, and C. 143-13 recognizes epitope region B, which is located at residue 3-104 of CD25 and can effectively block IL-2 binding to CD25. 143-13 was assigned to CD25 at the Fourth International Workshop (code A27).

Positive control: PHA-stimulated human lymphocytes, human lymph nodes and tonsils.

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