anti-CD99 mouse monoclonal, EBS-CD-044, purified

Cat. No. 691611

Available, delivery time 1-7 days

€340.00*

Key Features
  • Mouse monoclonal
  • Suitable for FACS, ICC/IF, IHC and WB
  • Reacts with human
  • Isotype: IgM kappa
Product description
Quantity 1 ml (100 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgM kappa
Conjugate Unconjugated
Application FACS, ICC/IF, IHC, WB
Purification Affinity chromatography
Reactivity Human
Storage 2-8°C
Intended use Research use only
Clone EBS-CD-044
Immunogen Purified E-rosette forming cells from human PBL
Concentration 100 µg/ml
Formulation PBS with 0.02% sodium azide
UniprotID P14209 (Human)
Synonym CD99 antigen, 12E7, E2 antigen, Protein MIC2, T-cell surface glycoprotein E2, CD antigen CD99, CD99, MIC2, MIC2X, MIC2Y
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:50-1:100 (1-2 µg/ml)
Immunohistochemistry (IHC) - frozen 1:50-1:100 (1-2 µg/ml)
Immunohistochemistry (IHC) - paraffin 1:25-1:50 (2-4 µg/ml; microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
Flow Cytometry (FACS) 1-2 µg/million cells in 0.1 ml; fix cells in 4% PFA for 10 min at 4°C, permeabilize with 0.02% saponin or digitonin for 15 min at 4°C
Western Blot (WB) 1:50-1:100 (1-2 µg/ml)
Background

EBS-CD-044 reacts with CD99 or MIC2. Human thymocytes, PBLs and some T-ALL isolates and cell lines are positive. It is also present on pancreas and on Ewing sarcoma, which forms the practical application of the antibody. It is involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes.

Positive control: pancreas, thymus, Ewing sarcoma.

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