anti-Calcitonin Gene Related Peptide rabbit polyclonal, serum
- Rabbit polyclonal
- Suitable for ICC/IF and IHC
- Reacts with human and rat
|Quantity||50 µl (lyoph.)|
|Storage before reconstitution||2-8°C until indicated expiry date|
|Storage after reconstitution||Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles|
|Intended use||Research use only|
|Immunogen||Calcitonin gene-related peptide (synthetic, rat, #6006, Peninsula), conjugated to BSA|
|Formulation||Lyophilized; reconstitute in 100 µl dist. water|
|Synonym||Calcitonin gene-related peptide 1, Alpha-type CGRP, Calcitonin gene-related peptide I, CGRP-I, CALCA, CALC1|
|Tested applications||Tested dilutions|
|Immunocytochemistry (ICC)/ Immunofluorescence (IF)||1:800-1:1,500|
|Immunohistochemistry (IHC) - frozen||1:1,000-1:2,000|
|Immunohistochemistry (IHC) - paraffin||1:1,000-1:2,000 (microwave treatment recommended)|
The CGRP-sequence was predicted from the corresponding mRNA. CGRP is present in the C-cells of the thyroid and in nerves, in both brain and periphery, particularly within the sensory system. CGRP is a potent vasodilator, probably involved in neurogenic inflammation. Medullary carcinomas of the thyroid contain large amounts of CGRP. Absorption with 10-100 ug CGRP per ml diluted antiserum abolishes the staining while calcitonin, substance P and Neurokinin A do not. The antiserum cross-reacts with amylin.
Positive control: frozen sections of rat colon.
Q & A's
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The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at email@example.com.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.