anti-Chlamydia Multiepitope Cocktail mouse monoclonal, ACI, ICK, 502, FITC Conjugate
- Mouse monoclonal
- Suitable for ICC/IF and IHC
- Reacts with Chlamydia genus
- Isotype: IgG1 and IgG3
|IgG1 and IgG3
|Research use only
|ACI, ICK, 502
|Lyophilized; reconstitute in 1.5 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4); contains Evans blue as counterstain
|Immunocytochemistry (ICC)/ Immunofluorescence (IF)
|Immunohistochemistry (IHC) - frozen
|Immunohistochemistry (IHC) - paraffin
The multi-epitope cocktail detects the elementary bodies (Ebs), reticulate bodies (RBs), intermediate forms, chlamydial inclusions and specific cell-associated anti-gen(s) directly in samples. The conjugate is used for direct immunofluorescence staining combining three specific monoclonal antibodies (mab) conjugated to fluo-rescein isothiocyanate (FITC).
One mab is specific for the genus-specific epitope located on the Chlamydia LPS and identifies all the 15 known serovars of C. trachomatis as well as C. psittaci and C. pneumoniae by displaying bright fluores-cence of intracellular inclusions and pin-point shaped extracellular organisms as well as free cell-associated chlamydial antigen(s).
A second mab reacts with an epitope on the Chlamydia-outer membrane complex protein (60 kDa) and shows preferential fluorescence around the periphery of individual RBs of all serotypes of C. trachomatis and of C. psittaci strains; this antibody also stains the EBs of C-complex serovars as pin-point shaped structures.
The third mab identifies a species-specific epitope on the major outer membrane protein (40 kDa) and shows brilliant fluorescence with EBs, RBs and cytoplasmic inclusions of B-complex serovars of C. trachomatis, whereas C-complex serovars show medium to weak fluorescence.
The combination of these 3 specific mabs provides uniform and intense staining of all the stages in the developmental cycle of the known C. trachomatis serovars (A-C, D-K, L1-L3) and C. psittaci and C. pneumoniae strains. A positive cross-reactivity was also reported for Chlamydophila pecorum.
Contains Evans blue as counterstain.
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The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.