Epitope mapped
Disease related

anti-Chlamydia Multiepitope Cocktail mouse monoclonal, ACI, ICK, 502, FITC Conjugate

Cat. No. FR97457

Available, delivery time 1-7 days


Key Features
  • Mouse monoclonal
  • Suitable for ICC/IF and IHC
  • Reacts with Chlamydia genus
  • Isotype: IgG1 and IgG3
Product description
Quantity 1.5 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgG1 and IgG3
Conjugate FITC
Application ICC/IF, IHC
Purification Affinity chromatography
Reactivity Chlamydia genus
Storage 2-8°C
Intended use Research use only
Clone ACI, ICK, 502
Immunogen Chlamydia antigen
Formulation Lyophilized; reconstitute in 1.5 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4); contains Evans blue as counterstain
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Ready-to-use
Immunohistochemistry (IHC) - frozen Ready-to-use
Immunohistochemistry (IHC) - paraffin Ready-to-use

The multi-epitope cocktail detects the elementary bodies (Ebs), reticulate bodies (RBs), intermediate forms, chlamydial inclusions and specific cell-associated anti-gen(s) directly in samples. The conjugate is used for direct immunofluorescence staining combining three specific monoclonal antibodies (mab) conjugated to fluo-rescein isothiocyanate (FITC).
One mab is specific for the genus-specific epitope located on the Chlamydia LPS and identifies all the 15 known serovars of C. trachomatis as well as C. psittaci and C. pneumoniae by displaying bright fluores-cence of intracellular inclusions and pin-point shaped extracellular organisms as well as free cell-associated chlamydial antigen(s).
A second mab reacts with an epitope on the Chlamydia-outer membrane complex protein (60 kDa) and shows preferential fluorescence around the periphery of individual RBs of all serotypes of C. trachomatis and of C. psittaci strains; this antibody also stains the EBs of C-complex serovars as pin-point shaped structures.
The third mab identifies a species-specific epitope on the major outer membrane protein (40 kDa) and shows brilliant fluorescence with EBs, RBs and cytoplasmic inclusions of B-complex serovars of C. trachomatis, whereas C-complex serovars show medium to weak fluorescence.
The combination of these 3 specific mabs provides uniform and intense staining of all the stages in the developmental cycle of the known C. trachomatis serovars (A-C, D-K, L1-L3) and C. psittaci and C. pneumoniae strains. A positive cross-reactivity was also reported for Chlamydophila pecorum.

Contains Evans blue as counterstain.

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Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.

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