anti-Cingulin guinea pig polyclonal, serum

Cat. No. GP26

Available, delivery time 1-7 days


Key Features
  • Guinea pig polyclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with bovine, human, mouse and rabbit
Product description
Quantity 100 µl
Antibody Type Polyclonal
Host Guinea pig
Conjugate Unconjugated
Application ICC/IF, IHC, WB
Purification Stabilized antiserum
Reactivity Bovine, Human, Mouse, Rabbit
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Immunogen Full length GST-fusion protein of human cingulin
Formulation Contains 0.09% sodium azide and 0.5% BSA
UniprotID P23805 (Bovine),Q9P2M7 (Human),P59242 (Mouse),B7NZD9 (Rabbit)
Synonym Cingulin, CGN, KIAA1319
Note Centrifuge prior to opening
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:100 (initial fixation with formaldehyde recommended for optimal staining)
Immunohistochemistry (IHC) - frozen assay dependent
Immunohistochemistry (IHC) - paraffin Assay dependent (microwave treatment recommended)
Western Blot (WB) Assay dependent

The antibody reacts specifically with cingulin (a ca.150 kD polypeptide) present in tight junctions (zonula occludens) of polar epithelia (e.g. colon, duodenum, kidney, liver, pancreas) of transitional epithelia (e.g. bladder epithelium) and of stratified epithelia (e.g. skin, foot sole, tongue, oesophagus, vagina).
It is negative with endothelia of vessels and capillaries (e.g. in heart and brain).

Polypeptide reacting: Cingulin, a polypeptide of 126.5 kD (calculated from aa sequence data); apparent Mr 150 kD (after SDS-PAGE); pI 6.5 (very similar to that of symplekin, another junctional protein).

Reactivity on cultured cell lines: Carcinoma cell lines of human(e.g. MCF-7, Caco-2, PLC, A-431), bovine (MDBK, BMGE) and canine (MDCK) origin.

Positive control: total cell lysate of e.g. CaCo-2 cells.

References/Publications (2)
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