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anti-Collagen Type IV rabbit polyclonal, serum

human Lung
human Small Intestine (paraffin)
human Aorta
human Liver
human Aorta
human Lung
human Lung
Involvement of the Reck tumor suppressor protein in maternal and embryonic vascular remodeling in mice.
Chandana, E. P., Maeda, Y., et al. Involvement of the Reck tumor suppressor protein in maternal and embryonic vascular remodeling in mice. BMC Dev Biol. 2010-08-06.

Species/Reactant: Mus musculus (House mouse)
Applications: Immunohistochemistry

Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
Involvement of the Reck tumor suppressor protein in maternal and embryonic vascular remodeling in mice.
Chandana, E. P., Maeda, Y., et al. Involvement of the Reck tumor suppressor protein in maternal and embryonic vascular remodeling in mice. BMC Dev Biol. 2010-08-06.

Species/Reactant: Mus musculus (House mouse)
Applications: Immunohistochemistry

Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
Activation of Src mediates PDGF-induced Smad1 phosphorylation and contributes to the progression of glomerulosclerosis in glomerulonephritis.
Mima, A., Abe, H., et al. Activation of Src mediates PDGF-induced Smad1 phosphorylation and contributes to the progression of glomerulosclerosis in glomerulonephritis. PLoS One. 2011-03-30.

Species/Reactant: Mus musculus (House mouse)
Applications: Western Blotting

Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
Activation of Src mediates PDGF-induced Smad1 phosphorylation and contributes to the progression of glomerulosclerosis in glomerulonephritis.
Mima, A., Abe, H., et al. Activation of Src mediates PDGF-induced Smad1 phosphorylation and contributes to the progression of glomerulosclerosis in glomerulonephritis. PLoS One. 2011-03-30.

Species/Reactant: Mus musculus (House mouse)
Applications: Western Blotting

Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
Activation of Src mediates PDGF-induced Smad1 phosphorylation and contributes to the progression of glomerulosclerosis in glomerulonephritis.
Mima, A., Abe, H., et al. Activation of Src mediates PDGF-induced Smad1 phosphorylation and contributes to the progression of glomerulosclerosis in glomerulonephritis. PLoS One. 2011-03-30.

Species/Reactant: Mus musculus (House mouse)
Applications: Western Blotting

Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
Activation of Src mediates PDGF-induced Smad1 phosphorylation and contributes to the progression of glomerulosclerosis in glomerulonephritis.
Mima, A., Abe, H., et al. Activation of Src mediates PDGF-induced Smad1 phosphorylation and contributes to the progression of glomerulosclerosis in glomerulonephritis. PLoS One. 2011-03-30.

Species/Reactant: Mus musculus (House mouse)
Applications: Western Blotting

Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
Activation of Src mediates PDGF-induced Smad1 phosphorylation and contributes to the progression of glomerulosclerosis in glomerulonephritis.
Mima, A., Abe, H., et al. Activation of Src mediates PDGF-induced Smad1 phosphorylation and contributes to the progression of glomerulosclerosis in glomerulonephritis. PLoS One. 2011-03-30.

Species/Reactant: Mus musculus (House mouse)
Applications: Western Blotting

Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
Activation of Src mediates PDGF-induced Smad1 phosphorylation and contributes to the progression of glomerulosclerosis in glomerulonephritis.
Mima, A., Abe, H., et al. Activation of Src mediates PDGF-induced Smad1 phosphorylation and contributes to the progression of glomerulosclerosis in glomerulonephritis. PLoS One. 2011-03-30.

Species/Reactant: Mus musculus (House mouse)
Applications: Western Blotting

Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
Activation of Src mediates PDGF-induced Smad1 phosphorylation and contributes to the progression of glomerulosclerosis in glomerulonephritis.
Mima, A., Abe, H., et al. Activation of Src mediates PDGF-induced Smad1 phosphorylation and contributes to the progression of glomerulosclerosis in glomerulonephritis. PLoS One. 2011-03-30.

Species/Reactant: Mus musculus (House mouse)
Applications: Western Blotting

Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
Activation of Src mediates PDGF-induced Smad1 phosphorylation and contributes to the progression of glomerulosclerosis in glomerulonephritis.
Mima, A., Abe, H., et al. Activation of Src mediates PDGF-induced Smad1 phosphorylation and contributes to the progression of glomerulosclerosis in glomerulonephritis. PLoS One. 2011-03-30.

Species/Reactant: Mus musculus (House mouse)
Applications: Western Blotting

Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
Activation of Src mediates PDGF-induced Smad1 phosphorylation and contributes to the progression of glomerulosclerosis in glomerulonephritis.
Mima, A., Abe, H., et al. Activation of Src mediates PDGF-induced Smad1 phosphorylation and contributes to the progression of glomerulosclerosis in glomerulonephritis. PLoS One. 2011-03-30.

Species/Reactant: Mus musculus (House mouse)
Applications: Western Blotting

Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
Inferring Growth Control Mechanisms in Growing Multi-cellular Spheroids of NSCLC Cells from Spatial-Temporal Image Data.
Jagiella, N., Müller, B., et al. Inferring Growth Control Mechanisms in Growing Multi-cellular Spheroids of NSCLC Cells from Spatial-Temporal Image Data. PLoS Comput Biol. 2016-02-01.

Species/Reactant: Homo sapiens (Human)
Applications: Immunocytochemistry-immunofluorescence

Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
Inferring Growth Control Mechanisms in Growing Multi-cellular Spheroids of NSCLC Cells from Spatial-Temporal Image Data.
Jagiella, N., Müller, B., et al. Inferring Growth Control Mechanisms in Growing Multi-cellular Spheroids of NSCLC Cells from Spatial-Temporal Image Data. PLoS Comput Biol. 2016-02-01.

Species/Reactant: Homo sapiens (Human)
Applications: Immunocytochemistry-immunofluorescence

Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
Hepatitis B virus surface proteins accelerate cholestatic injury and tumor progression in Abcb4-knockout mice.
Zahner, D., Glimm, H., et al. Hepatitis B virus surface proteins accelerate cholestatic injury and tumor progression in Abcb4-knockout mice. Oncotarget. 2017-08-08.

Species/Reactant: Mus musculus (House mouse)
Applications: Immunohistochemistry

Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
Involvement of the Reck tumor suppressor protein in maternal and embryonic vascular remodeling in mice.
Involvement of the Reck tumor suppressor protein in maternal and embryonic vascular remodeling in mice.
Activation of Src mediates PDGF-induced Smad1 phosphorylation and contributes to the progression of glomerulosclerosis in glomerulonephritis.
Activation of Src mediates PDGF-induced Smad1 phosphorylation and contributes to the progression of glomerulosclerosis in glomerulonephritis.
Activation of Src mediates PDGF-induced Smad1 phosphorylation and contributes to the progression of glomerulosclerosis in glomerulonephritis.
Activation of Src mediates PDGF-induced Smad1 phosphorylation and contributes to the progression of glomerulosclerosis in glomerulonephritis.
Activation of Src mediates PDGF-induced Smad1 phosphorylation and contributes to the progression of glomerulosclerosis in glomerulonephritis.
Activation of Src mediates PDGF-induced Smad1 phosphorylation and contributes to the progression of glomerulosclerosis in glomerulonephritis.
Activation of Src mediates PDGF-induced Smad1 phosphorylation and contributes to the progression of glomerulosclerosis in glomerulonephritis.
Activation of Src mediates PDGF-induced Smad1 phosphorylation and contributes to the progression of glomerulosclerosis in glomerulonephritis.
Activation of Src mediates PDGF-induced Smad1 phosphorylation and contributes to the progression of glomerulosclerosis in glomerulonephritis.
Inferring Growth Control Mechanisms in Growing Multi-cellular Spheroids of NSCLC Cells from Spatial-Temporal Image Data.
Inferring Growth Control Mechanisms in Growing Multi-cellular Spheroids of NSCLC Cells from Spatial-Temporal Image Data.
Hepatitis B virus surface proteins accelerate cholestatic injury and tumor progression in Abcb4-knockout mice.
Cat. No. 10760

Prices excl. VAT

Available, delivery time: 1-7 days

€241.00*

Datasheet
Key Features
  • Rabbit polyclonal
  • Suitable for IHC
  • Reacts with humans, mouse and rat
Product description
Quantity 250 µl
Antibody Type Polyclonal
Host Rabbit
Conjugate Unconjugated
Application IHC
Purification Stabilized antiserum
Reactivity Human, Mouse, Rat
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Immunogen Isolated from human placenta
Formulation Contains 0.09% sodium azide
UniprotID P02462 (Human),P02463 (Mouse),F1MA59 (Rat)
Synonym Collagen alpha-1(IV chain [Cleaved into: Arresten], COL4A1
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:50 - 1:100
Immunohistochemistry (IHC) - paraffin 1:50 - 1:100 (proteolytic treatment required)
Background

The antibody is reactive with collagen type IV of basement membranes, and shows a homogeneous staining pattern in all tissues. As neoplastic cells of invasive carcinomas often lack a continuous basement membrane, the antiserum is useful to distinguish between non-invasive and invasive lesions. Additionally, it can be used for the differentiation of bullous lesions in dermatopathology. In immunohistochemistry no cross-reactivity with other collagens at optimal dilutions. In immunoblotting, a slight cross-reactivity with collagen type V is observed.

Positive control: Skin, kidney.

References/Publications (9)
Publication
Species
Application
Publication Markus, M. A., Ferrari, D. P., Alves, F. & Ramos-Gomes, F. Effect of tissue fixation on the optical properties of structural components assessed by non-linear microscopy imaging. Biomed Opt Express 14, 3988 (2023).
Species mouse
Application IHC
Publication Li, H. et al. RECK in Neural Precursor Cells Plays a Critical Role in Mouse Forebrain Angiogenesis. iScience. 19, 559-571 (2019)
Species mouse
Application IHC-IF (frozen)
Publication Domke, L and Franke, W. The cell-cell junctions of mammalian testes.., Cell Tissue Res, 375, 451-482, (2019)
Species bovine
Application WB
Publication Olaru, F. et al. Intracapillary immune complexes recruit and activate slan-expressing CD16+ monocytes in human lupus nephritis. JCI.Insight. 3,  (2018).
Species mouse
Application IHC-IF (frozen)
Publication Zahner, D. et al. Hepatitis B virus surface proteins accelerate cholestatic injury and tumor progression in Abcb4-knockout mice. Oncotarget. 8, 52560-52570 (2017).
Species mouse
Application IHC (paraffin)
Publication Taromi, S. et al. Proteasome inhibitor bortezomib enhances the effect of standard chemotherapy in small cell lung cancer. Oncotarget. 8, 97061-97078 (2017).
Species human
Application IHC (paraffin)
Publication Jagiella, N., Müller, B., Müller, M., Vignon-Clementel, I. E. & Drasdo, D. Inferring Growth Control Mechanisms in Growing Multi-cellular Spheroids of NSCLC Cells from Spatial-Temporal Image Data. PLoS Comput. Biol. (2016).
Species human
Application IHC (frozen)
Publication Chandana, E. P. S. et al. Involvement of the Reck tumor suppressor protein in maternal and embryonic vascular remodeling in mice. BMC Dev. Biol. 10, 84 (2010).
Species mouse
Application IHC (paraffin)
Publication Oh, J. et al. Mutations in two matrix metalloproteinase genes, MMP-2 and MT1-MMP, are synthetic lethal in mice. Oncogene 23, 5041–5048 (2004).
Species mouse
Application IHC (paraffin)
Downloads
Datasheet
MSDS
FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.

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  • Mouse monoclonal
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  • Isotype: IgG2b

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