Disease related

anti-Complement C3a alpha mouse monoclonal, H13, lyophilized, purified

Cat. No. 61018

Available, delivery time 1-7 days

€211.00*

Key Features
  • Mouse monoclonal
  • Suitable for ELISA, IHC and WB
  • Reacts with guinea pig, human and rabbit
  • Isotype: IgG2b
Product description
Quantity 50 µg
Antibody Type Monoclonal
Host Mouse
Isotype IgG2b
Conjugate Unconjugated
Application ELISA, IHC, WB
Purification Affinity chromatography
Reactivity Guinea pig, Human, Rabbit
Storage before reconstitution 2-8°C until indicated expiry date
Storage after reconstitution Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone H13
Immunogen Human complement component C3
Concentration 50 µg/ml after reconstitution with 1ml dist. water

Formulation Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:10
ELISA Assay dependent
Western Blot (WB) Assay dependent
Background

H13 recognizes a C3a-determinant (eg C3 alpha-chain) which is present also on native C3. H13 allows discrimination of ubiquitous or nonspecifically bound native C3 from biologically active C3b fragment; C3b or C are negative with H13
Polypeptide reacting: C3a-determinant on C3 alpha-chain
Specific Application: Detection of C3 deposits in tissue, cells, microorganisms and immune complexes. It should be used with positive control anti-C3b (H11/H206). Non-activated, absorbed C3 contains the C3a determinant, whereas activated C3 is negative with H13.

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