Epitope mapped

anti-Complement C3b alpha mouse monoclonal, H206, lyophilized, purified

Cat. No. 61019

Available, delivery time 1-7 days

€207.00*

Key Features
  • Mouse monoclonal
  • Suitable for ELISA, IHC and WB
  • Reacts with human
  • Isotype: IgG1
Product description
Quantity 50 µg
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application ELISA, IHC, WB
Purification Affinity chromatography
Reactivity Human
Storage before reconstitution 2-8°C until indicated expiry date
Storage after reconstitution Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone H206
Immunogen Human complement component C3
Concentration 50 µg/ml after reconstitution with 1 ml dist. water
Formulation Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:10
ELISA Assay dependent
Western Blot (WB) Assay dependent
Background

H 206 allows demonstration of C3 deposits in tissue, on cells, on microorganisms and in immune complexes. It does not react with C3a or C3d and does not inhibit hemolytic function of C3. The epitope was located on the C-termius of the alpha-chain (39.5 kD fragment of C3c).

Disorders specifically detected: Detection of C3b deposits in tissue, on cells, on microorganisms and in immune complexes. It should be used with positive control anti C3b-ß, (H11; Cat. No. 61020) and negative control anti-C3a (H13; Cat. No. 61018). Activated, deposited C3b bears C3b-a and C3b-ß, but no longer C3a. Ubiquitous or nonspecifically absorbed C3 is still C3a positive with H13.

Polypeptide reacting: C3b alpha-chain.

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