Disease related

anti-Cyclin D1 mouse monoclonal, DCS-6, liquid, purified

Liquid

Liquid

Lyophilized

Cat. No. 690053

Available, delivery time 1-7 days

€350.00*

Key Features
  • Purified, liquid
  • Mouse monoclonal
  • Suitable for FACS, ICC/IF, IHC, IP, Neutralization Assay and WB
  • Reacts with dog, human, monkey, mouse and rat
  • Isotype: IgG2a
Product description
Quantity 1 ml (100 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG2a
Conjugate Unconjugated
Application FACS, ICC/IF, IHC, IP, Neutralization assay, WB
Purification Affinity chromatography
Reactivity Dog, Human, Monkey, Mouse, Rat
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone DCS-6
Immunogen Human recombinant full-length cyclin D1 protein
Concentration 100 µg/ml
Formulation 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4
UniprotID Q64HP0 (Dog, Canis familiaris),P24385 (Human),P25322 (Mouse),P39948 (Rat)
Synonym G1/S-specific cyclin-D1, B-cell lymphoma 1 protein, BCL-1, BCL-1 oncogene, PRAD1 oncogene, CCND1, BCL1, PRAD1
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunohistochemistry (IHC) - frozen 1:20-1:50
Immunohistochemistry (IHC) - paraffin 1:20-1:50 (microwave treatment recommended)
Flow Cytometry (FACS) Assay dependent
Immunoprecipitation (IP) Assay dependent
Neutralization Assay Assay dependent
Western Blot (WB) Assay dependent
Background

DCS-6 reacts specifically with cyclin D1 protein present predominantly in G1 phase of cell cycle; it does not cross-react with D2 or D3 cyclins (the closest relatives within the cyclin family); DCS-6 is especially useful in tumor diagnosis: it can be used as fast method to identify cases with 11q13 amplifications. Cyclin D1 gene is identical to the BCL-1 and the PRAD-1 oncogene and is part of the chromosome 11q13 amplicon which is amplified in several common tumor types like breast, lung, esophagus, urinary bladder and head and neck carcinoma. Polypeptide reacting: Mr 36 kDa polypeptide (cyclin D1 polypeptide of human cells).

Reactivity on cultured cell lines: MCF-7, MDA-MB-134 (breast cancer cell lines), UMSC22 (squamous cell carcinoma cell line), U-2-OS (osteosarcoma cell line) RT 112, A 431, HT 29.

References/Publications (5)
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