+49 (0)6221 8278-0
info@progen.com
Go to homepage

anti-Cytokeratin Type I & II, AE1/AE3, purified

Cat. No. 61835

Prices excl. VAT

Available, delivery time: 1-7 days

€350.00*

Datasheet
Key Features
  • Mouse monoclonal
  • Suitable for ICC/IF and IHC
  • Reacts with bovine, chicken, guinea pig, human, monkey, mouse, rabbit and rat
  • Isotype: IgG1
Product description
Quantity 1 ml (200 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application ICC/IF, IHC
Purification Affinity chromatography
Reactivity Bovine, Chicken, Guinea pig, Human, Monkey, Mouse, Rabbit, Rat
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone AE1/AE3
Immunogen Human epidermal keratin
Formulation 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunohistochemistry (IHC) - frozen 1:250-1:750
Immunohistochemistry (IHC) - paraffin 1:250-1:750 (protease treatment and/or microwave treatment recommended)
Background

AE1/AE3 represents an excellent marker for distinguishing carcinomas from non-epithelial tumors; reacts with all epithelium-derived tumors and their neoplasms. Decorates the majority of type I and type II keratins (formerly also designated cytokeratins).
Polypeptides reacting: Mr 40 kDa, Mr 48 kDa and Mr 50 kDa polypeptides of human epithelial proteins (keratins nos. K19, K16, K14) and most other acidic (type I) keratins (AE1); most basic (type II) keratins (Mr 52.5-68 kDa) of human epithelia (AE3)

References/Publications (10)
Publication
Species
Application
Publication Niwano, Y. et al. Protective effects of blue light-blocking shades on phototoxicity in human ocular surface cells. BMJ.Open.Ophthalmol. 4, e000217 (2019)
Species human
Application ICC-IF
Publication Chesa, P. G., Rettig, W. J. & Melamed, M. R. Expression of cytokeratins in normal and neoplastic colonic epithelial cells. Implications for cellular differentiation and carcinogenesis. Am. J. Surg. Pathol. 10, 829–835 (1986).
Species human
Application IHC
Publication Weiss, R. A., Eichner, R. & Sun, T. T. Monoclonal antibody analysis of keratin expression in epidermal diseases: A 48- and 56-kdalton keratin as molecular markers for hyperproliferative keratinocytes. J. Cell Biol. 98, 1397–1406 (1984).
Species human
Application WB
Publication Nelson, W. G., Battifora, H. & Sun, T. T. Specific Keratins as Molecular Markers for Neoplasms with a Stratified Epithelial Origin. Cancer Res. 44, 1600–1603 (1984).
Species human
Application WB
Publication Eichner, R., Bonitz, P. & Sun, T. T. Classification of epidermal keratins according to their immunoreactivity, isoelectric point, and mode of expression. J. Cell Biol. 98, 1388–1396 (1984).
Species human
Application IF,WB
Publication Shi, S. R. et al. Immunohistochemical study of nasopharyngeal carcinoma using monoclonal keratin antibodies. Am. J. Pathol. 117, 53–63 (1984).
Species human
Application IHC
Publication Nelson, W. G. & Sun, T. T. The 50- and 58-kdalton keratin classes as molecular markers for stratified squamous epithelia: Cell culture studies. J. Cell Biol. 97, 244–251 (1983).
Species human
Application IF,WB
Publication Sun, T. T. et al. Keratin classes: molecular markers for different types of epithelial differentiation. J Invest Dermatol 81, 109s–15s (1983).
Species human
Application IF,WB,IHC
Publication Tseng, S. C. G. et al. Correlation of specific keratins with different types of epithelial differentiation: Monoclonal antibody studies. Cell 30, 361–372 (1982).
Species human,monkey
Application IF,WB
Publication WOODCOCKMITCHELL, J., EICHNER, R., NELSON, W. G. & SUN, T. T. Immunolocalization of Keratin Polypeptides in Human-Epidermis Using Monoclonal-Antibodies. J. Cell Biol. 95, 580–588 (1982).
Species human
Application IF,WB,IHC
Downloads
Datasheet
MSDS
FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.

Contact

Need more product information?

Related Products

anti-Keratin K7 mouse monoclonal, Ks7.18, liquid, purified, sample
anti-Keratin K7 mouse monoclonal, Ks7.18, liquid, purified, sample
Cat. No. 690025S
Quantity: 200 µl (50 µg/ml)
  • Purified, liquid, small
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with bovine, human, pig and sheep
  • Isotype: IgG1

€79.00*
anti-Keratin K19 mouse monoclonal, Ks19.1 (A53-B/A2), liquid, purified, sample
anti-Keratin K19 mouse monoclonal, Ks19.1 (A53-B/A2), liquid, purified, sample
Cat. No. 690010S
Quantity: 200 µl (50 µg/ml)
  • Purified, liquid, small
  • Mouse monoclonal
  • Suitable for ELISA, ICC/IF, IHC and WB
  • Reacts with human
  • Isotype: IgG2a

€79.00*
anti-Keratin K20 mouse monoclonal, IT-Ks20.8, liquid, purified, sample
anti-Keratin K20 mouse monoclonal, IT-Ks20.8, liquid, purified, sample
Cat. No. 690026S
Quantity: 200 µl (50 µg/ml)
  • Purified, liquid, small
  • Mouse monoclonal
  • Suitable for IHC and WB
  • Reacts with human and mouse
  • Isotpye: IgG2a

€79.00*
anti-Keratin K8 mouse monoclonal, Ks8-17.2, supernatant
anti-Keratin K8 mouse monoclonal, Ks8-17.2, supernatant
Cat. No. 65130
  • Mouse monclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with bovine, hamster, human and rat
  • Isotype: IgG1

€143.00*
anti-Keratin K19 mouse monoclonal, Ks19.10, supernatant
anti-Keratin K19 mouse monoclonal, Ks19.10, supernatant
Cat. No. 65135
  • Mouse monoclonal
  • Suitable for ELISA, ICC/IF, IHC and WB
  • Reacts with bovine, human and rat
  • Isotype: IgG1

€143.00*
anti-Keratin K18 mouse monoclonal, Ks18.5, supernatant
anti-Keratin K18 mouse monoclonal, Ks18.5, supernatant
Cat. No. 65137
  • Mouse monoclonal
  • Suitable for ELISA, ICC/IF, IHC and WB
  • Reacts with bovine and human
  • Isotype: IgG1

€143.00*
anti-Keratin K2 mouse monoclonal, Ks2.342.7.4, supernatant
anti-Keratin K2 mouse monoclonal, Ks2.342.7.4, supernatant
Cat. No. 65191
  • Mouse monoclonal
  • Suitable for ICC/IF and IHC
  • Reacts with bovine and human
  • Isotype: IgG1

€147.00*
anti-Keratin K2 mouse monoclonal, Ks2.398.3.1, supernatant
anti-Keratin K2 mouse monoclonal, Ks2.398.3.1, supernatant
Cat. No. 65177
Quantity: 1 ml
  • Hybridoma cell culture supernatant
  • Mouse monoclonal
  • Suitable for IHC and WB
  • Reacts with human
  • Isotype: IgG1

€147.00*
anti-Keratin K5/K8 (Pan Epithelial) mouse monoclonal, C22, prediluted, purified
anti-Keratin K5/K8 (Pan Epithelial) mouse monoclonal, C22, prediluted, purified
Cat. No. 65031
Quantity: 5 ml
  • Purified, prediluted
  • Mouse monoclonal
  • Suitable for IHC and WB
  • Reacts with bovine, human, mouse and rat
  • Isotype: IgG1

Variants from €79.00*
€160.00*
anti-Keratin K7 mouse monoclonal, OVTL 12-30, supernatant
anti-Keratin K7 mouse monoclonal, OVTL 12-30, supernatant
Cat. No. 18315
  • Mouse monoclonal
  • Suitable for ICC/IF and IHC on frozen and paraffin sections
  • Reacts with human
  • Isotype: IgG1

€177.00*
anti-Keratin K7 + K17 mouse monoclonal, C46, supernatant
anti-Keratin K7 + K17 mouse monoclonal, C46, supernatant
Cat. No. 18313
  • Mouse monoclonal
  • Suitable for ICC/IF and IHC on frozen and paraffin sections
  • Reacts with bovine, dog, human and sheep
  • Isotype: IgG1

€177.00*
anti-Keratin K9 mouse monoclonal, Ks9.70 + Ks9.216, supernatant
anti-Keratin K9 mouse monoclonal, Ks9.70 + Ks9.216, supernatant
Cat. No. 651104
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with human
  • Isotypes: IgG1 and IgG3

€202.00*
anti-Keratin Pan mouse monoclonal, Lu-5, lyophilized, purified
anti-Keratin Pan mouse monoclonal, Lu-5, lyophilized, purified
Cat. No. 610145
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for IHC
  • Reacts with bovine, chicken, human, mouse, pleurodeles, rat, snake and xenopus
  • Isotype: IgG1

€202.00*
anti-Keratin K19 mouse monoclonal, Ks19.2 (Z105.6), liquid, purified
anti-Keratin K19 mouse monoclonal, Ks19.2 (Z105.6), liquid, purified
Cat. No. 690029
Quantity: 1 ml (50 µg/ml)
  • Purified, liquid
  • Mouse monoclonal
  • Suitable for ELISA, ICC/IF, IHC and WB
  • Reacts with bovine, human, rabbit, rat
  • Isotype: IgG2b

Variants from €79.00*
€207.00*
anti-Keratin K6 mouse monoclonal, KA12, lyophilized, purified
anti-Keratin K6 mouse monoclonal, KA12, lyophilized, purified
Cat. No. 61090
Quantity: 50 µg
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for IHC and WB
  • Reacts with human, mouse and rat
  • Isotype: IgG1

Variants from €79.00*
€207.00*
anti-Keratin K17 mouse monoclonal, Ks17.E3, liquid, purified
anti-Keratin K17 mouse monoclonal, Ks17.E3, liquid, purified
Cat. No. 690036
Quantity: 1 ml (50 µg/ml)
  • Purified, liquid
  • Mouse monoclonal
  • Suitable for IHC and WB
  • Reacts with human, rat
  • Isotype: IgG2b

Variants from €79.00*
€207.00*
anti-Keratin K8 mouse monoclonal, Ks8.7, liquid, purified
anti-Keratin K8 mouse monoclonal, Ks8.7, liquid, purified
Cat. No. 690038
Quantity: 1 ml (50 µg/ml)
  • Purified, liquid
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with hamster and human
  • Isotype: IgG1

Variants from €79.00*
€207.00*
anti-Keratin K13 mouse monoclonal, Ks13.1, lyophilized, purified
anti-Keratin K13 mouse monoclonal, Ks13.1, lyophilized, purified
Cat. No. 61007
Quantity: 50 µg
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with bovine, human, rat
  • Isotype: IgG1

Variants from €133.00*
€207.00*
anti-Keratin K18 mouse monoclonal, Ks18.27, lyophilized, purified
anti-Keratin K18 mouse monoclonal, Ks18.27, lyophilized, purified
Cat. No. 61034
  • Mouse monoclonal
  • Suitable for ELISA, IHC and WB
  • Reacts with bovine and human
  • Isotype: IgG1

€207.00*
anti-Keratin K18 Multiepitope Cocktail mouse monoclonal, Ks18.04 + 18.27 + 18.5 + 9B1 + 18.174, supernatant
anti-Keratin K18 Multiepitope Cocktail mouse monoclonal, Ks18.04 + 18.27 + 18.5 + 9B1 + 18.174, supernatant
Cat. No. 651134
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with human and mammals
  • Isotype: IgG1

€212.00*
anti-Keratin Pan rabbit polyclonal, purified
anti-Keratin Pan rabbit polyclonal, purified
Cat. No. 10550
  • Rabbit Polyclonal
  • Suitable for IHC on frozen and  paraffin sections
  • Reacts with human and mouse

€217.00*
anti-Keratin K10 mouse monoclonal, DE-K10, supernatant
anti-Keratin K10 mouse monoclonal, DE-K10, supernatant
Cat. No. 11414
Quantity: 1 ml
  • Hybridoma cell culture supernatant
  • Mouse monoclonal
  • Suitable for IHC on frozen and paraffin sections and WB
  • Reacts with human
  • Isotype: IgG1

€235.00*
anti-Keratin K18 mouse monoclonal, RCK106, supernatant
anti-Keratin K18 mouse monoclonal, RCK106, supernatant
Cat. No. 11416
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC on frozen sections and WB
  • Reacts with human, mouse and rabbit
  • Isotype: IgG1

€235.00*
anti-Keratin K18 mouse monoclonal, RGE53, supernatant
anti-Keratin K18 mouse monoclonal, RGE53, supernatant
Cat. No. 10500
  • Mouse monoclonal
  • Suitable for IHC on frozen sections and WB
  • Reacts with chicken, dog, human, mouse, pig, rabbit and rat
  • Isotype: IgG1

€235.00*
This website uses cookies to ensure the best experience possible. More information...