Disease related

anti-Desmocollin 1 mouse monoclonal, Dsc1-U100, lyophilized, purified

Lyophilized

Sample

Cat. No. 61092

Available, delivery time 1-7 days

€241.00*

Key Features
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for IHC and WB
  • Reacts with human, mouse, rat
  • Isotype: IgG1
Product description
Quantity 50 µg
Antibody Type Monoclonal
Isotype IgG1
Conjugate Unconjugated
Application IHC, WB
Purification Affinity chromatography
Reactivity Human, Mouse, Rat
Storage before reconstitution 2-8°C until indicated expiry date
Storage after reconstitution Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone Dsc1-U100
Immunogen Synthetic peptide corresponding to sequence present within the intracellular part of human desmocollin 1 (HQTLESVKGVGQGDTGRYATDWQC)
Concentration 50 µg/ml after reconstitution with 1 ml dist. water
Formulation Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
UniprotID
Synonym Desmocollin-1, DSC1, Cadherin family member 1, Desmosomal glycoprotein 2/3, DG2/DG3, CDHF1
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:50-1:100 (preincubation (directly after fixation) with 0.05-0.2% Triton X-100, for 5-10 min, depending on tissue type, is recommended; without detergent pretreatment a cytoplasmic component might cross-react)
Immunohistochemistry (IHC) - paraffin 1:50-1:100 (microwave treatment recommended; a slight crossreactivity with a lymphocyte subpopulation is abolished by mild trypsinization after the microwave step)
Western Blot (WB) Assay dependent
Background

Mouse monoclonal antibody localizes Dsc1 present in

  • Suprabasal layers of interfollicular epidermis
  • Specific cell layers (e.g. in hair follicle root sheath)
  • Hassall bodies (spheroidal formations of densely packed reticulum-derived cells in the thymus).

Reacts with 111 kDa (form "a") and 103 kDa (form "b") polypeptides (desmocollin 1 splice forms, both at pI 5.2) from human epidermal desmosomes.

Reactivity on cultured cell lines: positive on primary keratinocytes, however, restricted to local piles of differentiated cells. All simple epithelia are negative.

References/Publications (2)
Publication
Species
Application
Downloads
File
Category
Size
Filetype
Category Protocol
Size 924.86 KB
Filetype pdf
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€241.00*