Disease related
Frequently used

anti-Desmocollin 3 mouse monoclonal, Dsc3-U114, lyophilized, purified

Lyophilized

Sample

Cat. No. 61093

Available, delivery time 1-7 days

€241.00*

Key Features
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for IHC and WB
  • Reacts with human, mouse, rat
  • Isotype: IgG1
Product description
Quantity 50 µg
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application IHC, WB
Purification Affinity chromatography
Reactivity Human, Mouse, Rat
No reactivity Bovine
Storage before reconstitution 2-8°C until indicated expiry date
Storage after reconstitution Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone Dsc3-U114
Immunogen Synthetic peptide corresponding to a sequence present on the extracellular anchor domain of human desmocollin 3 (VHGAPFYFSLPNTSPEISRLWSLTKVC)
Concentration 50 µg/ml after reconstitution with 1 ml dist. water
Formulation Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
UniprotID Q14574 (Human),P55292 (Mouse),D3ZZI6 (Rat)
Synonym Desmocollin-3, Cadherin family member 3, Desmocollin-4, HT-CP, DSC3, CDHF3, DSC4
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:10-1:100 (for better resolution preincubation (directly after fixation) with 0.05-0.2% Triton X-100, for 5-10 min, depending on tissue type, is recommended)
Immunohistochemistry (IHC) - paraffin 1:10-1:100 (microwave treatment recommended)
Western Blot (WB) Assay dependent
Background

The mouse monoclonal antibody localizes Dsc3 present in

  • All living epidermal layers
  • In glandular duct cells
  • In basal matrix cells and outer root sheath of hair follicles
  • Basal and suprabasal cell layers of stratified epithelia (e.g. vagina, tongue, esophagus), basal layer of bladder urothelium and complex epithelium of trachea
  • Thymic reticulum cells

All simple epithelia are negative. Intercalated disks of myocardium are
negative. Polypeptide reacting: 109 kDa and 100 kDa polypeptides (desmocollin 3 splice isoforms at pI 5.2) from human epidermal desmosomes.
Reactivity on cultured cell lines: most cultured cell lines derived from stratified squamous epithelia or squamous cell carcinomas are positive (e.g. A-431, HaCaT).

References/Publications (7)
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Category Protocol
Size 924.86 KB
Filetype pdf
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anti-Desmocollin 1 mouse monoclonal, Dsc1-U100, lyophilized, purified
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Quantity: 50 µg
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for IHC and WB
  • Reacts with human, mouse, rat
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€241.00*