Disease related
Frequently used

anti-Desmocollin 3 mouse monoclonal, Dsc3-U114, purified, sample (ready-to-use)

Lyophilized

Sample

Cat. No. 61093S

Available, delivery time 1-7 days

€49.00*

Key Features
  • Purified, prediluted sample, ready-to-use for IHC
  • Mouse monoclonal
  • Suitable for IHC and WB
  • Reacts with human, mouse, rat
  • Isotype: IgG1
Product description
Quantity 600 µl (ready-to-use)
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application IHC, WB
Purification Affinity chromatography
Reactivity Human, Mouse, Rat
No reactivity Bovine
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone Dsc3-U114
Immunogen Synthetic peptide corresponding to a sequence present on the extracellular anchor domain of human desmocollin 3 (VHGAPFYFSLPNTSPEISRLWSLTKVC)
Formulation PBS pH 7.4 with 0.5% BSA and 0.09% sodium azide
UniprotID Q14574 (Human),P55292 (Mouse),D3ZZI6 (Rat)
Synonym Desmocollin-3, Cadherin family member 3, Desmocollin-4, HT-CP, DSC3, CDHF3, DSC4
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen Ready-to-use (for better resolution preincubation (directly after fixation) with 0.05-0.2% Triton X-100, for 5-10 min, depending on tissue type, is recommended)
Immunohistochemistry (IHC) - paraffin Ready-to-use (microwave treatment recommended)
Western Blot (WB) Assay dependent
Background

The mouse monoclonal antibody localizes Dsc3 present in

  • All living epidermal layers
  • In glandular duct cells
  • In basal matrix cells and outer root sheath of hair follicles
  • Basal and suprabasal cell layers of stratified epithelia (e.g. vagina, tongue, esophagus), basal layer of bladder urothelium and complex epithelium of trachea
  • Thymic reticulum cells

All simple epithelia are negative. Intercalated disks of myocardium are
negative. Polypeptide reacting: 109 kDa and 100 kDa polypeptides (desmocollin 3 splice isoforms at pI 5.2) from human epidermal desmosomes.
Reactivity on cultured cell lines: most cultured cell lines derived from stratified squamous epithelia or squamous cell carcinomas are positive (e.g. A-431, HaCaT).

References/Publications (7)
Downloads
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Category
Size
Filetype
Category Protocol
Size 924.86 KB
Filetype pdf
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