anti-Desmoglein 2 mouse monoclonal, 10G11, lyophilized, purified

Cat. No. 61059

Available, delivery time 1-7 days

€238.00*

Key Features
  • Mouse monclonal
  • Suitable for ELISA, IHC and WB
  • Reacts with human
  • Isotype: IgG1
Product description
Quantity 50 µg
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application ELISA, IHC, WB
Purification Affinity chromatography
Reactivity Human
No reactivity Mouse, Rat
Storage before reconstitution 2-8°C until indicated expiry date
Storage after reconstitution Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone 10G11
Immunogen Recombinant peptide (220aa of 2nd and 3rd extracellular repeat domain)
Concentration 50 µg/ml after reconstitution with 1 ml dist. water
Formulation Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
UniprotID Q14126 (Human)
Synonym Desmoglein-2, Cadherin family member 5, HDGC, DSG2, CDHF5
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:10 (For better resolution preincubation (directly after fixation) with 0.05-0.2% Triton X-100 for 5-10 min, depending on tissue type, is recommended (Schäfer S. et al., 1996))
ELISA Assay dependent
Western Blot (WB) Assay dependent
Background

Antibody recognizes desmoglein 2 present in low concentrations in all epithelia and in tumors derived therefrom as well as in heart muscle and dendritic reticulum cells of lymphatic follicles.
In basal epithelial cells desmoglein 2 expression is generally very low. Polypeptide reacting: desmoglein 2 (1069aa; calculated mol. wt. 116,760)
Reactivity on cultured cell lines: Many, including CaCo-2, HaCaT, MCF-7

References/Publications (2)
Publication
Species
Application
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