anti-Desmoglein 2 rabbit polyclonal, serum

Cat. No. 610121

Available, delivery time 1-7 days

€240.00*

Key Features
  • Rabbit polyclonal
  • Suitable for ELISA, IHC and WB
  • Reacts with human and mouse
Product description
Quantity 100 µl
Antibody Type Polyclonal
Host Rabbit
Conjugate Unconjugated
Application ELISA, IHC, WB
Purification Stabilized antiserum
Reactivity Human, Mouse
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Immunogen Recombinant peptide (extracellular repeat domain E2 of human Desmoglein 2)
Formulation Contains 0.09% sodium azide
UniprotID Q14126 (Human),O55111 (Mouse)
Synonym Desmoglein-2, Cadherin family member 5, HDGC, DSG2, CDHF5
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:100 (preincubation (directly after fixation with -20°C methanol) with 0.05-0.5% Triton X-100, for 5-10 min, depending on tissue type, recommended)
ELISA Assay dependent
Western Blot (WB) 1:3,000
Background

Antibody recognizes desmoglein 2 present in all epithelia and in tumors derived therefrom as well as in heart muscle and dendritic reticulum cells of lymphatic follicles. In basal epithelial cells desmoglein 2 expression is generally very low.

Polypeptide reacting: Desmoglein 2 (1069aa; calculated mol. wt. 116,760)

Reactivity on cultured cell lines: Many, including CaCo-2, HaCaT, MCF-7.

References/Publications (4)
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