anti-Desmoglein 2 rabbit polyclonal, serum

Cat. No. 610121

Available, delivery time: 1-7 days

€240.00*

Key Features

Rabbit polyclonal
Suitable for ELISA, IHC and WB
Reacts with human and mouse

Product description

Quantity	100 µl
Antibody Type	Polyclonal
Host	Rabbit
Conjugate	Unconjugated
Application	ELISA, IHC, WB
Purification	Stabilized antiserum
Reactivity	Human, Mouse
Storage	Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use	Research use only
Immunogen	Recombinant peptide (extracellular repeat domain E2 of human Desmoglein 2)
Formulation	Contains 0.09% sodium azide
UniprotID	Q14126 (Human),O55111 (Mouse)
Synonym	Desmoglein-2, Cadherin family member 5, HDGC, DSG2, CDHF5
Note	Centrifuge prior to opening

Applications

Tested applications	Tested dilutions
Immunohistochemistry (IHC) - frozen	1:100 (preincubation (directly after fixation with -20°C methanol) with 0.05-0.5% Triton X-100, for 5-10 min, depending on tissue type, recommended)
ELISA	Assay dependent
Western Blot (WB)	1:3,000

Background

Antibody recognizes desmoglein 2 present in all epithelia and in tumors derived therefrom as well as in heart muscle and dendritic reticulum cells of lymphatic follicles. In basal epithelial cells desmoglein 2 expression is generally very low.

Polypeptide reacting: Desmoglein 2 (1069aa; calculated mol. wt. 116,760)

Reactivity on cultured cell lines: Many, including CaCo-2, HaCaT, MCF-7.

References/Publications (9)

Publication

Species

Application

Publication Shoykhet, M., Waschke, J. & Yeruva, S. Cardiomyocyte cohesion is increased after ADAM17 inhibition. Front Cell Dev Biol 11, (2023).

Species mouse

Application WB, ICC-IF

Publication Yeruva, S. et al. Cholinergic signaling impairs cardiomyocyte cohesion. Acta Physiologica 236, (2022).

Species mouse

Publication Wanuske, M. T. et al. Clustering of desmosomal cadherins by desmoplakin is essential for cell-cell adhesion., Acta Physiol (Oxf) 231, e13609, (2021).

Species human

Application ICC-IF

Publication Yeruva, S. et al. Adrenergic Signaling-Induced Ultrastructural Strengthening of Intercalated Discs via Plakoglobin Is Crucial for Positive Adhesiotropy in Murine Cardiomyocytes. Front Physiol 11, 526845 (2020).

Species mouse

Application WB

Publication Schinner, C. et al. Stabilization of desmoglein-2 binding rescues arrhythmia in arrhythmogenic cardiomyopathy. JCI.Insight. 5, (2020)

Species mouse

Application WB

Publication Valenzuela-Iglesias, A. et al. Desmoglein 1 regulates invadopodia by suppressing EGFR/ERK signaling in an ERBIN-dependent manner. Molecular Cancer Research 17, 1195–1206 (2019).

Species human

Application WB

Publication Domke, L and Franke, W. The cell-cell junctions of mammalian testes.., Cell Tissue Res, 375, 451-482, (2019)

Species pig

Application ICC-IF

Publication Nekrasova, O. et al. Desmosomal cadherin association with Tctex-1 and cortactin-Arp2/3 drives perijunctional actin polymerization to promote keratinocyte delamination. Nat.Commun. 9, 1053 (2018).

Species human

Application WB,ICC-IF

Publication Pieperhoff, S. & Franke, W. W. The area composita of adhering junctions connecting heart muscle cells of vertebrates - IV: coalescence and amalgamation of desmosomal and adhaerens junction components - late processes in mammalian heart development. Eur.

Species mouse

Application IHC (frozen)

Downloads

Datasheet

MSDS

FAQs

The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.

Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.

The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet.

PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.

Homogenization of tissue in PBS (supplemented with protease inhibitors).
Centrifugation at 10E4 x g.
Optional: treat pellet with DNase.
Resuspend pellet in detergent buffer (e.g. 1% Triton X-100), stir for 1 h at 2-8°C.
Centrifugation at 10E4 x g.
Resuspend pellet in high salt buffer (with 1.5 M KCl), stir for 1 h at 2-8°C.
Centrifugation at 10E4 x g.
Resuspend pellet in low salt buffer (e.g. 50 mM NaCl), stir for 30 min at 2-8°C.
Centrifugation at 10E4 x g: pellet contains raw cytoskeletal fraction.

Transfer from a low concentration acrylamide gel (e.g. 8%).
Reduce the methanol concentration of the transfer buffer (limit is 50% of the routine concentration 20% methanol).
Use the wet transfer method and transfer at least 4 h (or better overnight) at 100-200 mA.
In order to prevent the accumulation of gas bubbles, stir the transfer buffer continuously during the transfer (e.g. with a magnetic stirrer).
If possible use a cytoskeletal preparation of your material to be analyzed (in the case of cultured cells additionally DNase treatment) rather than whole cell lysate.
As a general rule we would like to recommend loading that much protein into one gel lane that the intermediate filament polypeptides (in the 40-60 kD range) can be readily detected in a control gel stained with Coomassie blue.

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