Highly published

anti-Desmoplakin 1/2 mouse monoclonal, DP1&2-2.15; DP1-2.17; DP1-2.20, supernatant

Cat. No: 65146

Prices excl. tax

Available, delivery time 1-7 days

$246.00*

Datasheet

Key Features

Mouse monoclonal
Suitable for IHC and WB
Reacts with bovine, chicken, dog, human, mouse, pig and rat
Isotype: IgG1

Product description

Quantity	5 ml
Antibody Type	Monoclonal
Host	Mouse
Isotype	IgG1
Conjugate	Unconjugated
Application	IHC, WB
Purification	Hybridoma cell culture supernatant
Reactivity	Bovine, Chicken, Dog, Human, Mouse, Pig, Rat
Storage	Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use	Research use only
Clone	DP1&2-2.15; DP1-2.17; DP1-2.20
Immunogen	Bovine desmoplakins 1&2
Formulation	Contains 0.09% sodium azide
UniprotID	A0A3Q1MR22 (Bovine),E1BWI0 (Chicken),A0A5F4CNG7 (Dog, Canis familiaris),P15924 (Human),E9Q557 (Mouse),Q8VBY1 (Rat)
Synonym	Desmoplakin, DP, 250/210 kDa paraneoplastic pemphigus antigen, DSP

Applications

Tested applications	Tested dilutions
Immunohistochemistry (IHC) - frozen	Ready-to-use
Immunohistochemistry (IHC) - paraffin	Ready-to-use (microwave treatment recommended)
Western Blot (WB)	Assay dependent

Background

The antibody cocktail shows distinct punctate membrane staining of different epithelia and the aerea composite of the myocard. Tumors specifically detected: primary and metastatic cancer; meningioma.

Polypeptides reacting: Desmoplakins 1 and 2 (Mr 250,000 and 215,000).

Tested cultured cell lines: several human carcinoma cell lines: A-431, BMGE, MDCK.

References/Publications (33)

Publication

Species

Application

Publication Erman, A. et al. How Cancer Cells Invade Bladder Epithelium and Form Tumors: The Mouse Bladder Tumor Model as a Model of Tumor Recurrence in Patients. Int. J. Mol. Sci. 22, 6328 (2021).

Species mouse

Application IHC-IF

Publication Atsugi, T. et al. Holocrine Secretion Occurs outside the Tight Junction Barrier in Multicellular Glands: Lessons from Claudin-1-Deficient Mice. J.Invest.Dermatol. , (2019)

Species mouse

Application IHC-IF (frozen)

Publication Domke, L and Franke, W. The cell-cell junctions of mammalian testes.., Cell Tissue Res, 375, 451-482, (2019)

Species pig

Application ICC-IF

Publication Abreu-Velez, A. et al. Involvement of the Areae Compositae of the Heart in Endemic Pemphigus Foliaceus. Dermatol.Pract.Concept. 9, 181-186 (2019)

Species human

Application IHC-IF (frozen)

Publication Neutzner, A. et al. A perfusion bioreactor-based 3D model of the subarachnoid space based on a meningeal tissue construct. Fluids.Barriers.CNS. 16, 17 (2019)

Species human

Application IHC (paraffin)

Publication Polivka, L. et al. Epithelial barrier dysfunction in desmoglein-1 deficiency. J.Allergy.Clin.Immunol. 142, 702-706.e7 (2018).

Species human

Application IHC (paraffin)

Publication Abreu-Velez, A. et al. Subclinical oral involvement in patients with endemic pemphigus foliaceus. Dermatol.Pract.Concept. 8, 252-261 (2018).

Species human

Application IHC-IF (frozen)

Publication Abreu-Velez, A. et al. Patients affected by endemic pemphigus foliaceus in Colombia, South America exhibit autoantibodies to optic nerve sheath envelope cell junctions. Dermatol.Pract.Concept. 8, 1-6 (2018).

Species human

Application ICC-IF

Publication Dedeić, Z. et al. Cell autonomous role of iASPP deficiency in causing cardiocutaneous disorders. Cell.Death.Differ. 25, 1289-1303 (2018)

Species mouse

Application ICC-IF

Publication Zeleny, T. N. C., Kohler, C., Neutzner, A., Killer, H. E. & Meyer, P. Cell–Cell Interaction Proteins (Gap Junctions, Tight Junctions, and Desmosomes) and Water Transporter Aquaporin 4 ... Front. Neurol. 8, 308 (2017).

Species human

Application IHC

Publication Kant, S., Holthöfer, B., Magin, T. M., Krusche, C. A. & Leube, R. E. Desmoglein 2-Dependent Arrhythmogenic Cardiomyopathy Is Caused by a Loss of Adhesive Function. Circ. Cardiovasc. Genet. 8, 553–63 (2015).

Species mouse

Application IHC (paraffin)

Publication Franke, W. W. et al. Protein LUMA is a cytoplasmic plaque constituent of various epithelial adherens junctions and composite junctions of myocardial intercalated disks: a unifying finding for cell biology and cardiology. Cell Tissue Res. 357, 159–72 (2

Species human,pig

Application IHC (frozen),ICC-IF

Publication Fischer-Kešo, R. et al. Plakophilins 1 and 3 bind to FXR1 and thereby influence the mRNA stability of desmosomal proteins. Mol. Cell. Biol. 34, 4244–56 (2014).

Species human

Application WB,IP

Publication Langbein, L. et al. New facets of keratin K77: Interspecies variations of expression and different intracellular location in embryonic and adult skin of humans and mice. Cell Tissue Res. 354, 793–812 (2013).

Species human

Application IHC (frozen)

Publication Ibi, M. et al. Trichoplein controls microtubule anchoring at the centrosome by binding to Odf2 and ninein. J Cell Sci 124, 857–864 (2011).

Species mouse

Application ICC-IF

Publication Langbein, L., Eckhart, L., Rogers, M. A., Praetzel-Wunder, S. & Schweizer, J. Against the rules: Human keratin K80 - Two functional alternative splice variants, K80 and K80.1, with special cellular localization in a wide range of epithelia. J. Biol. Chem

Species human

Application IHC (frozen),IEM

Publication Rickelt, S., Winter-Simanowski, S., Noffz, E., Kuhn, C. & Franke, W. W. Upregulation of plakophilin-2 and its acquisition to adherens junctions identifies a novel molecular ensemble of cell-cell-attachment characteristic for transformed mesenchymal cells

Species human

Application WB

Publication Walter, B., Schlechter, T., Hergt, M., Berger, I. & Hofmann, I. Differential expression pattern of protein ARVCF in nephron segments of human and mouse kidney. Histochem. Cell Biol. 130, 943–956 (2008).

Species human

Application IHC (frozen)

Publication Pieperhoff, S. & Franke, W. W. The area composita of adhering junctions connecting heart muscle cells of vertebrates - IV: coalescence and amalgamation of desmosomal and adhaerens junction components - late processes in mammalian heart development. Eur.

Species mouse

Application IHC (frozen),IEM

Publication Bortfeld, M. et al. Human multidrug resistance protein 8 (MRP8/ABCC11), an apical efflux pump for steroid sulfates, is an axonal protein of the CNS and peripheral nervous system. Neuroscience 137, 1247–1257 (2006).

Species dog

Application ICC-IF

Publication Franke, W. W., Borrmann, C. M., Grund, C. & Pieperhoff, S. The area composita of adhering junctions connecting heart muscle cells of vertebrates. I. Molecular definition in intercalated disks of cardiomyocytes by immunoelectron microscopy of desmosomal p

Species human,mouse,rat

Application IEM

Publication Borrmann, C. M. et al. The area composita of adhering junctions connecting heart muscle cells of vertebrates. II. Colocalizations of desmosomal and fascia adhaerens molecules in the intercalated disk. Eur. J. Cell Biol. 85, 469–485 (2006).

Species human,mouse,rat,bovine,eel,carp,xenopus

Application WB,IHC (frozen)

Publication Kartenbeck, J., Haselmann, U. & Gassler, N. Synthesis of junctional proteins in metastasizing colon cancer cells. Eur. J. Cell Biol. 84, 417–430 (2005).

Species human

Application WB

Publication Silberberg, M., Charron, A. J., Bacallao, R. & Wandinger-Ness, A. Mispolarization of Desmosomal Proteins and Altered Cell Adhesion in Autosomal Dominant Polycystic Kidney Disease. Am J Physiol Ren. Physiol. 288, (2005).

Species human

Application ICC-IF

Publication Koeser, J., Troyanovsky, S. M., Grund, C. & Franke, W. W. De novo formation of desmosomes in cultured cells upon transfection of genes encoding specific desmosomal components. Exp. Cell Res. 285, 114–30 (2003).

Species human

Application WB,ICC-IF

Publication Akat, K. et al. Molecular characterization of desmosomes in meningiomas and arachnoidal tissue. Acta Neuropathol. 106, 337–347 (2003).

Species human

Application WB,IHC (frozen)

Publication Langbein, L. et al. Tight junction-related structures in the absence of a lumen: occludin, claudins and tight junction plaque proteins in densely packed cell formations of stratified epithelia and squamous cell carcinomas. Eur. J. Cell Biol. 82, 385–40

Species bovine

Application IHC (frozen)

Publication Langbein, L. et al. Tight junctions and compositionally related junctional structures in mammalian stratified epithelia and cell cultures derived therefrom. Eur. J. Cell Biol. 81, 419–435 (2002).

Species bovine

Application IHC (frozen)

Publication Hofmann, I. et al. Interaction of plakophilins with desmoplakin and intermediate filament proteins: an in vitro analysis. J. Cell Sci. 113, 2471–83 (2000).

Species human

Application IP

Publication Hatzfeld, M., Haffner, C., Schulze, K. & Vinzens, U. The Function of Plakophilin 1 in Desmosome Assembly and Actin Filament Organization. J. Cell Biol. 149, 209–222 (2000)

Species human

Application ICC-IF

Publication Chitaev, N. A. et al. The Binding of Plakoglobin to Desmosomal Cadherins: Patterns of Binding Sites and Topogenic Potential. J. Cell Biol. 113, 359–369 (1996).

Species human

Application ICC-IF

Publication Owaribe, K. et al. The hemidesmosomal plaque. I. Characterization of a major constituent protein as a differentiation marker for certain forms of epithelia. Differentiation. 45, 207–20 (1990).

Species bovine

Application IHC (frozen)

Publication Cowin, P., Kapprell, H.-P. & Franke, W. W. The Complement of Desmosomal Plaque Proteins in Different Cell Types. J. Cell Biol. 101, 1442–1454 (1985)

Species bovine

Application WB,IHC (frozen)

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Datasheet

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FAQs

The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

PROGEN offers different formats for mouse monoclonal antibodies. Different antibodies may not be offered in each format, the following are all possible formats:

Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet.
Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet.
Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.

We offer bulk amounts starting from 1 mg for many of our mouse monoclonal or recombinant antibodies. Please contact us for further information or use the “bulk button” on the product page.

Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.

The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet.

PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.

Wash cells 1x with PBS (37°C).
Fix in ice-cold (pre-cooled to -20°C) methanol (5-10 min), followed by ice-cold acetone (30-60 sec).
Optional: to enhance the epitope accessibility incubate for 5 min with 0.1% Triton-X100 in PBS.
Let air dry.
Block with the serum of the species in which the secondary antibody was raised for 30 min at RT.
Incubate with primary antibody overnight at 4°C.
Wash 3x with PBS at RT.
Incubate 1 h with fluorochrome-conjugated secondary antibody.
Wash 2x with PBS.
Embed in mounting medium.

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anti-Desmoplakin 1/2 mouse monoclonal, DP1&2-2.15; DP1-2.17; DP1-2.20, supernatant

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Protocol for ICC with Desmoplakin 1/2 antibodies