Desmoplakin 1 staining on A431 cells (courtesy of The Human Protein Atlas, www.proteinatlas.org, Thul PJ et al, 2017. A subcellular map of the human proteome. Science)
Desmoplakin 1 staining on human skin (courtesy of The Human Protein Atlas, www.proteinatlas.org, Thul PJ et al, 2017. A subcellular map of the human proteome. Science)
Desmoplakin 1 staining on A431 cells (courtesy of The Human Protein Atlas, www.proteinatlas.org, Thul PJ et al, 2017. A subcellular map of the human proteome. Science)
Desmoplakin 1 staining on human skin (courtesy of The Human Protein Atlas, www.proteinatlas.org, Thul PJ et al, 2017. A subcellular map of the human proteome. Science)
Key Features
- Mouse monoclonal
- Suitable for IHC and WB
- Reacts with bovine, chicken, human, mouse and rat
- Isotype: IgG1
Product description
| Quantity | 50 µg |
|---|---|
| Antibody Type | Monoclonal |
| Host | Mouse |
| Isotype | IgG1 |
| Conjugate | Unconjugated |
| Application | IHC, WB |
| Purification | Affinity chromatography |
| Reactivity | Bovine, Chicken, Human, Mouse, Rat |
| Storage before reconstitution | 2-8°C until indicated expiry date |
| Storage after reconstitution | Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
| Intended use | Research use only |
| Clone | DP2.17 |
| Immunogen | Bovine desmoplakin 1 |
| Concentration | 50 µg/ml after reconstitution with 1 ml dist. water |
| Formulation | Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4) |
| UniprotID | A0A3Q1MR22 (Bovine),E1BWI0 (Chicken),P15924 (Human),E9Q557 (Mouse),Q8VBY1 (Rat) |
| Synonym | Desmoplakin, DP, 250/210 kDa paraneoplastic pemphigus antigen, DSP |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunohistochemistry (IHC) - frozen | 1:10 |
| Immunohistochemistry (IHC) - paraffin | 1:10 (microwave treatment recommended) |
| Western Blot (WB) | Assay dependent |
Background
DP2.17 shows distinct punctate membrane staining of epithelia. DP2.17 reacts with the rod fragment of desmoplakin 1.
Polypeptide reacting: Desmoplakin no. 1 (Mr 250,000 polypeptide of desmosomal plaques)
Reactivity on cultured cell lines: Several human carcinoma cell lines, such as MCF-7, A-431, TR-146; bovine cells: MDBK, BMGE
Tumors specifically detected: Primary and metastatic carcinoma; meningioma
References/Publications (2)
Publication
Species
Application
Species
mouse
Application
WB,ICC-IF
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FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Please find the solvent for reconstitution and the amount of liquid for reconstitution on the antibody datasheet. The buffer formulation after reconstitution is also mentioned in the datasheet.
PROGEN offers different formats for mouse monoclonal antibodies. Different antibodies may not be offered in each format, the following are all possible formats:
- Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
- Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet.
- Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet.
- Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.
Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
The expiration date of our antibodies is indicated on the product label.
We offer bulk amounts starting from 1 mg for many of our mouse monoclonal or recombinant antibodies. Please contact us for further information or use the “bulk button” on the product page.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet.
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.
- Wash cells 1x with PBS (37°C).
- Fix in ice-cold (pre-cooled to -20°C) methanol (5-10 min), followed by ice-cold acetone (30-60 sec).
- Optional: to enhance the epitope accessibility incubate for 5 min with 0.1% Triton-X100 in PBS.
- Let air dry.
- Block with the serum of the species in which the secondary antibody was raised for 30 min at RT.
- Incubate with primary antibody overnight at 4°C.
- Wash 3x with PBS at RT.
- Incubate 1 h with fluorochrome-conjugated secondary antibody.
- Wash 2x with PBS.
- Embed in mounting medium.
- Homogenization of tissue in PBS (supplemented with protease inhibitors).
- Centrifugation at 10E4 x g.
- Optional: treat pellet with DNase.
- Resuspend pellet in detergent buffer (e.g. 1% Triton X-100), stir for 1 h at 2-8°C.
- Centrifugation at 10E4 x g.
- Resuspend pellet in high salt buffer (with 1.5 M KCl), stir for 1 h at 2-8°C.
- Centrifugation at 10E4 x g.
- Resuspend pellet in low salt buffer (e.g. 50 mM NaCl), stir for 30 min at 2-8°C.
- Centrifugation at 10E4 x g: pellet contains raw cytoskeletal fraction.
- Transfer from a low concentration acrylamide gel (e.g. 8%).
- Reduce the methanol concentration of the transfer buffer (limit is 50% of the routine concentration 20% methanol).
- Use the wet transfer method and transfer at least 4 h (or better overnight) at 100-200 mA.
- In order to prevent the accumulation of gas bubbles, stir the transfer buffer continuously during the transfer (e.g. with a magnetic stirrer).
- If possible use a cytoskeletal preparation of your material to be analyzed (in the case of cultured cells additionally DNase treatment) rather than whole cell lysate.
- As a general rule we would like to recommend loading that much protein into one gel lane that the intermediate filament polypeptides (in the 40-60 kD range) can be readily detected in a control gel stained with Coomassie blue.
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