anti-Drebrin guinea pig polyclonal, serum

Cat. No. GP254

Available, delivery time 1-7 days

€274.00*

Key Features
  • Guinea pig polyclonal
  • Suitable for ICC/IF, IHC, IP and WB
  • Reacts with bovine, human, mouse and rat
Product description
Quantity 100 µl
Antibody Type Polyclonal
Host Guinea pig
Conjugate Unconjugated
Application ICC/IF, IHC, IP, WB
Purification Stabilized antiserum
Reactivity Bovine, Human, Mouse, Rat
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Immunogen Synthetic human peptide (aa 254-272) coupled to KLH
Formulation Contains 0.09% sodium azide
UniprotID A1L5A7 (Bovine),Q16643 (Human),Q9QXS6 (Mouse),Q07266 (Rat)
Synonym Drebrin, Developmentally-regulated brain protein, DBN1, D0S117E
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunohistochemistry (IHC) - frozen 1:100
Immunohistochemistry (IHC) - paraffin 1:100 (microwave treatment recommended)
Immunoprecipitation (IP) Assay dependent
Western Blot (WB) Assay dependent
Background

The antiserum specifically reacts with drebrin, a widespread actin-associating protein of 70 kD (SDS-PAGE mobility shows Mr 120,000). Drebrin has originally been described only in neuronal cells, but has been recently found also in the actin-rich lamellipodia and filopodia of motile cells. It is enriched at junctional plaques, defining a specific microfilament anchorage system in polar epithelial cells.
Positive staining was found in many tissues, including diverse epithelia and carcinoma (e.g. in epidermis basal cells are positive, whereas overlying cells are negative; also positive: regenerating epithelium during wound healing; basal cell carcinoma; skin melanoma), specific types of endothelia and smooth muscle; especially prominent in the mesangial cells of renal glomeruli and in the Sertoli cells of testis.
Also positive: epithelia of hair follicles and eccrine sweat glands. Consistently negative were, however, hepatocytes and cross-striated muscle.
Positive cultured cell lines: PLC, U333, HUVEC, HaCat, HeLa, MCF-7, SV-80, B1; B16 (mouse melanoma cells)

References/Publications (3)
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