Disease related
Frequently used

anti-EP-CAM mouse monoclonal, HEA125, lyophilized, purified





Cat. No. 61004

Available, delivery time 1-7 days


Key Features
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with human
  • Isotype: IgG1
Product description
Quantity 50 µg
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application FACS, ICC/IF, IHC, WB
Purification Affinity chromatography
Reactivity Human
No reactivity Mouse
Storage before reconstitution 2-8°C until indicated expiry date
Storage after reconstitution Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone HEA125
Immunogen HT-29 colon carcinoma cell line
Concentration 50 µg/ml after reconstitution with 1 ml dist. water
Formulation Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Synonym Human Epithelium Antigen, HEA, 17-1A, EPG34, CD326, TACSTD1
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunohistochemistry (IHC) - frozen 1:10-1:50 (1-5 µg/ml)
Immunohistochemistry (IHC) - paraffin 1:10-1:50 (1-5 µg/ml; protease treatment recommended)
Flow Cytometry (FACS) Assay dependent
Western Blot (WB) Assay dependent (non-reducing conditions)

The Ep-CAM (HEA125) antibody recognizes the epithelial cell adhesion molecule Ep-CAM (also described as 17-1A antigen, EPG34, CD326, and TACSTD1). This antigen is widely expressed on cells of epithelial origin and tumors derived therefrom. HEA125 represents an excellent marker to discriminate epithelial from mesothelial structures. The antigen has been detected in all carcinoma types tested (18 different origins). A subset of squamous cell carcinoma is negative.

The antibody can be used as alternative to the anti-EpCAM antibody clone BerEp4.

Polypeptide reacting: Mr 40,000 human epithelium-specific cell surface glycoprotein (Ep-CAM).

Reactivity on cultured cell lines: all carcinoma cell lines tested so far; particularly strong reaction with colon carcinoma cell lines.

References/Publications (8)
Category Protocol
Size 924.86 KB
Filetype pdf
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