anti-Enkephalin rabbit polyclonal, serum

Cat. No. 16020

Available, delivery time 1-7 days

€252.00*

Key Features
  • Rabbit Polyclonal
  • Suitable for IHC on frozen and paraffin sections
  • Reacts with cat, pig, rat
Product description
Quantity 50 µl (lyoph.)
Antibody Type Polyclonal
Host Rabbit
Conjugate Unconjugated
Application IHC
Purification Undiluted antiserum
Reactivity Cat, Pig, Rat
Storage before reconstitution 2-8°C until indicated expiry date
Storage after reconstitution Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Immunogen Synthetic met-enkephalin (Sigma), conjugated to BSA
Formulation Lyophilized; reconstitute in 100 µl dist. water
UniprotID P04094 (Rat)
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:400-1:800
Immunohistochemistry (IHC) - paraffin 1:400-1:800 (microwave treatment recommended)
Background

Enkephalins are small peptides derived from large precursers (pro-enkephalin A and B) containing multiple enkephalin copies. They are the most abundant opioid peptides in the body and are widely distributed in the brain and the peripheral nervous system and occur also in the adrenal medulla. Several types of neuroendocrine tumors, incl. pheochromocytomas, neuroblastomas and bronchial and gastrointestinal endocrine tumors, produce enkephalin.
Absorption with 10-100 ug immunogen per ml diluted antiserum abolishes the staining, while beta-endorphin does not. Cross-reacts with leu-enkephalin.

Positive control: Frozen sections of cat or pig small intestine.

References/Publications (3)
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