anti-Enkephalin rabbit polyclonal, serum
- Rabbit Polyclonal
- Suitable for IHC on frozen and paraffin sections
- Reacts with cat, pig, rat
|Quantity||50 µl (lyoph.)|
|Reactivity||Cat, Pig, Rat|
|Storage before reconstitution||2-8°C until indicated expiry date|
|Storage after reconstitution||Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles|
|Intended use||Research use only|
|Immunogen||Synthetic met-enkephalin (Sigma), conjugated to BSA|
|Formulation||Lyophilized; reconstitute in 100 µl dist. water|
|Tested applications||Tested dilutions|
|Immunohistochemistry (IHC) - frozen||1:400-1:800|
|Immunohistochemistry (IHC) - paraffin||1:400-1:800 (microwave treatment recommended)|
Enkephalins are small peptides derived from large precursers (pro-enkephalin A and B) containing multiple enkephalin copies. They are the most abundant opioid peptides in the body and are widely distributed in the brain and the peripheral nervous system and occur also in the adrenal medulla. Several types of neuroendocrine tumors, incl. pheochromocytomas, neuroblastomas and bronchial and gastrointestinal endocrine tumors, produce enkephalin.
Absorption with 10-100 ug immunogen per ml diluted antiserum abolishes the staining, while beta-endorphin does not. Cross-reacts with leu-enkephalin.
Positive control: Frozen sections of cat or pig small intestine.
Q & A's
0 of 0 reviews