anti-Estrogen-Receptor mouse monoclonal, ER-70, purified

Cat. No. 691684

Available, delivery time 1-7 days

€340.00*

Key Features
  • Mouse monoclonal
  • Suitable for IHC
  • Reacts with human
  • Isotype IgG1 kappa
Product description
Quantity 1 ml (100 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG1 kappa
Conjugate Unconjugated
Application IHC
Purification Affinity chromatography
Reactivity Human
Storage 2-8°C
Intended use Research use only
Clone ER-70
Immunogen Recombinant human estrogen receptor alpha protein (aa2-85)
Concentration 100 µg/ml
Formulation PBS with 0.02% sodium azide
UniprotID P03372 (Human)
Synonym Estrogen receptor, ER, ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1, ESR1, ESR, NR3A1
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:25-1:50 (2-4 µg/ml)
Immunohistochemistry (IHC) - paraffin 1:25-1:50 (2-4 µg/ml; microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
Background

ER-70 is specific to ER alpha (67 kDa) and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates are increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. Structurally ER consists of 6 functional domains (domain A-F). Functional mapping of the estrogen receptor has determined a transcriptional promoting activity in the A/B domain. The hormone-binding domain (E domain) is located towards the carboxy terminal, whereas the DNA-binding domain (C-domain) is found in the central portion of the molecule. It has been speculated that the presence in breast cancer cells of truncated forms of estrogen receptor lacking the hormone-binding domain might promote the uncontrolled growth of the tumor. The ER-70 epitope is located in the transcriptional promoting (A/B) domain of ER).

Positive control: Human uterus, ER positive breast cancer, MCF-7 cells.

Downloads
Q & A's

Please enter a valid email address.
Please enter a valid question.
Fields marked with * are mandatory.

There aren't any asked questions yet.
Customer Reviews

0 of 0 reviews

Leave a review!

Share your experiences with other customers.