- Rabbit polyclonal
- Suitable for ICC/IF and IHC on frozen and paraffin sections
- Reacts with hamster, human, pig, rat
Product description
| Quantity | 50 µl (lyoph.) |
|---|---|
| Antibody Type | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Application | ICC/IF, IHC |
| Purification | Undiluted antiserum |
| Reactivity | Hamster, Human, Pig, Rat |
| Storage before reconstitution | 2-8°C until indicated expiry date |
| Storage after reconstitution | Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
| Intended use | Research use only |
| Immunogen | Porcine pancreatic glucagon (Novo, Denmark), conjugated to BSA |
| Formulation | Lyophilized; reconstitute in 100 µl dist. water |
| UniprotID | P01275 (Human),P01274 (Pig),P05110 (Guinea pig),P06883 (Rat) |
| Synonym | Pro-glucagon [Cleaved into: Glicentin; Glicentin-related polypeptide, GRPP; Oxyntomodulin, OXM, OXY; Glucagon; Glucagon-like peptide 1, GLP-1, Incretin hormone; Glucagon-like peptide 1(7-37, GLP-1(7-37; Glucagon-like peptide 1(7-36, GLP-1(7-36; Glucagon-like peptide 2, GLP-2], GCG |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | 1:1,000-1:3,000 |
| Immunohistochemistry (IHC) - frozen | 1:1,000-1:3,000 |
| Immunohistochemistry (IHC) - paraffin | 1:1,000-1:3,000 (microwave treatment recommended) |
Background
Glicentin contains the glucagon sequence and is produced in a prominent population of endocrine cells in the distal intestine as well as in pancreatic glucagon cells and in the nerves in the brain. Serum levels of glicentin are elevated after food uptake and in certain clinical conditions, e.g. after resections of the intestine. The functional role of glicentin is largely unknown. Glicentin occurs in endocrine tumors arising in the distal intestine (rectal carcinoids) and in pancreatic islet cell tumors.
Absorption with 10-100 ug glucagon and glicentin per ml diluted antiserum abolishes the staining, while secretin, GIP and VIP do not.
Positive control: formalin-fixed paraffin sections of pig pancreas.
References/Publications (2)
FAQs
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
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