anti-Glucagon rabbit polyclonal, serum

human Pancreas Alpha-Cells

Cat. No. 11184

Prices excl. VAT

Available, delivery time: 1-7 days

€235.00*

Datasheet

Key Features

Rabbit polyclonal
Suitable for IHC on frozen and paraffin sections
Reacts with human, rat

Product description

Quantity	250 µl
Antibody Type	Polyclonal
Host	Rabbit
Conjugate	Unconjugated
Application	IHC
Purification	Stabilized antiserum
Reactivity	Human, Rat
Storage	Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use	Research use only
Immunogen	Porcine pancreatic glucagon/BSA
Formulation	Contains 0.09% sodium azide
UniprotID	P01275 (Human),P06883 (Rat)
Synonym	Pro-glucagon [Cleaved into: Glicentin; Glicentin-related polypeptide, GRPP; Oxyntomodulin, OXM, OXY; Glucagon; Glucagon-like peptide 1, GLP-1, Incretin hormone; Glucagon-like peptide 1(7-37, GLP-1(7-37; Glucagon-like peptide 1(7-36, GLP-1(7-36; Glucagon-like peptide 2, GLP-2], GCG
Note	Centrifuge prior to opening

Applications

Tested applications	Tested dilutions
Immunohistochemistry (IHC) - frozen	1:50 (no proteolytic treatment required)
Immunohistochemistry (IHC) - paraffin	1:50 (microwave treatment recommended; no proteolytic treatment required)

Background

The antibody is specific to pancreatic glucagon. Glucagon is produced by the alpha-cells of the pancreatic islets. It occurs also in endocrine cells of the gut and in neurons in the brain. Glucagon is a common constituent of endocrine pancreatic tumours and of rectal carcinoids, such tumours may give rise to the so called glucagonoma syndrome. The antigen localization is cytoplasmic.
Positive control: Pancreas.

References/Publications (3)

Publication

Species

Application

Publication Myrsén, U., Ahrén, B. & Sundler, F. Neuropeptide Y is expressed in subpopulations of insulin- and non-insulin-producing islet cells in the rat after dexamethasone treatment: a combined immunocytochemical and in situ hybridisation study. Regul. Pept. 60

Species rat

Application IHC (frozen)

Publication Larsson, L., Alumets, J., Håkanson, R., Simonsson, M. & Sundler, F. Carcinoid granule extract contains antigenic determinants common to peptide hormone-producing cells and endocrine tumours. Virchows Arch. A. Pathol. Anat. Histopathol. 416, 465–72 (19

Species human

Application IHC (frozen)

Publication Alumets, J. et al. Neurohormonal peptides in endocrine tumors of the pancreas, stomach, and upper small intestine: I. An immunohistochemical study of 27 cases. Ultrastruct. Pathol. 5, 55–72 (1983).

Species human

Application IHC (paraffin)

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Datasheet

MSDS

FAQs

The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.

Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.

The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet.

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