Key Features
- Rabbit polyclonal
- Suitable for IHC on frozen and paraffin sections
- Reacts with human, rat
Product description
Quantity | 250 µl |
---|---|
Antibody Type | Polyclonal |
Host | Rabbit |
Conjugate | Unconjugated |
Application | IHC |
Purification | Stabilized antiserum |
Reactivity | Human, Rat |
Storage | Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
Intended use | Research use only |
Immunogen | Porcine pancreatic glucagon/BSA |
Formulation | Contains 0.09% sodium azide |
UniprotID | P01275 (Human),P06883 (Rat) |
Synonym | Pro-glucagon [Cleaved into: Glicentin; Glicentin-related polypeptide, GRPP; Oxyntomodulin, OXM, OXY; Glucagon; Glucagon-like peptide 1, GLP-1, Incretin hormone; Glucagon-like peptide 1(7-37, GLP-1(7-37; Glucagon-like peptide 1(7-36, GLP-1(7-36; Glucagon-like peptide 2, GLP-2], GCG |
Note | Centrifuge prior to opening |
Applications
Tested applications | Tested dilutions |
---|---|
Immunohistochemistry (IHC) - frozen | 1:50 (no proteolytic treatment required) |
Immunohistochemistry (IHC) - paraffin | 1:50 (microwave treatment recommended; no proteolytic treatment required) |
Background
The antibody is specific to pancreatic glucagon. Glucagon is produced by the alpha-cells of the pancreatic islets. It occurs also in endocrine cells of the gut and in neurons in the brain. Glucagon is a common constituent of endocrine pancreatic tumours and of rectal carcinoids, such tumours may give rise to the so called glucagonoma syndrome. The antigen localization is cytoplasmic.
Positive control: Pancreas.
References/Publications (3)
Publication
Species
Application
Species
rat
Application
IHC (frozen)
Species
human
Application
IHC (frozen)
Species
human
Application
IHC (paraffin)
FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
The expiration date of our antibodies is indicated on the product label.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet.