Key Features
- Purified, lyophilized
- Mouse monoclonal
- Suitable for ICC/IF, IHC and WB
- Reacts with Puumala virus-infected cells
- Isotype: IgG1
Product description
| Quantity | 100 µg |
|---|---|
| Antibody Type | Monoclonal |
| Host | Mouse |
| Isotype | IgG1 |
| Conjugate | Unconjugated |
| Application | ICC/IF, IHC, WB |
| Purification | Affinity chromatography |
| Reactivity | Puumala virus-infected cells |
| Storage before reconstitution | 2-8°C until indicated expiry date |
| Storage after reconstitution | Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
| Intended use | Research use only |
| Clone | A1C5 |
| Immunogen | Recombinant Puumala nucleocapsid protein |
| Concentration | 100 µg/ml after reconstitution with 1 ml dist. water |
| Formulation | Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4) |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | 1:50 |
| Immunohistochemistry (IHC) - frozen | Assay dependent |
| Western Blot (WB) | Assay dependent |
Background
A1C5 reacts with an epitope present on the N-terminus (between aa 15 and 35) of the nucleocapsid protein (S segment) of hantavirus CG 18-20 strain (Puumala serotype)
and also cross-reacts with SNV (Sin Nombre virus) rN protein (Schmidt et al. 2005).
Schmidt, J., Meisel, H., Hjelle, B., Krueger, D. H. & Ulrich, R. Development and evaluation of serological assays for detection of human hantavirus infections caused by Sin Nombre virus. J. Clin. Virol. Off. Publ. Pan Am. Soc. Clin. Virol. 33, 247-53 (2005).
References/Publications (4)
Publication
Species
Application
Species
Hantavirus Hantaan,Hantavirus Puumala
Application
IF,WB
Species
Hantavirus Puumala
Application
WB
Species
Hantavirus Puumala
Application
WB
Species
Hantavirus Puumala
Application
ELISA
FAQs
Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
The expiration date of our antibodies is indicated on the product label.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet.
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.
Related Products
anti-Hantavirus Hantaan mouse monoclonal, B5D9, supernatant
€116.00*
Cat. No. B5D9-C
- Mouse monoclonal
- Suitable for ICC/IF and IHC
- Reacts with Hantaan virus-infected cells
- Isotype: IgG1
anti-Hantavirus Seoul mouse monoclonal, R31, supernatant
€165.00*
Cat. No. R31-C
- Mouse monclonal
- Suitable for ELISA, ICC/IF and IHC
- Reacts with Hantavirus Seoul strain R22
- Isotype: IgG1