anti-Histamine rabbit polyclonal, serum

Cat. No. 16043

Available, delivery time 1-7 days

€423.00*

Key Features
  • Rabbit polyclonal
  • Suitable for ICC/IF and IHC on frozen and paraffin sections
  • Reacts with human, rat
Product description
Quantity 50 µl (lyoph.)
Antibody Type Polyclonal
Host Rabbit
Conjugate Unconjugated
Application ICC/IF, IHC
Purification Undiluted antiserum
Reactivity Human, Rat
Storage before reconstitution 2-8°C until indicated expiry date
Storage after reconstitution Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Immunogen Histamine conjugated to HSA
Formulation Lyophilized; reconstitute in 100 µl dist. water
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:300-1:600
Immunohistochemistry (IHC) - frozen 1:1,500-1:2,000
Immunohistochemistry (IHC) - paraffin 1:1,500-1:2,000 (microwave treatment recommended)
Background

Histamine is a neurotransmitter in the central nervous system as well as a mast cell constituent. In addition, histamine is produced by endocrine cells (ECL-cells) in the oxynthic mucosa of the stomach. Absorption with 10-100 ug histamine per ml diluted antiserum abolishes the staining, while noradrenaline, 5-HT, VIP, glucagon and histidine do not.

Positive control: cryostat sections of carbodiimide fixed human skin or freeze-dried paraffin-sections (vapor fixed in diethylpyrocarbonate; DEPC) of rat stomach.

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