Highly published
anti-Histidine Decarboxylase rabbit polyclonal, serum
WB with anti-HDC antibody (Cat. No. 16045, 1:5000) and secondary anti-rabbit HRP antibody (Cat. No. 90003, 1:50,000), sample Raw246.7 whole cell lysate (6.7 µg)
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IHC of human skin (courtesy of J.Heß, University Hospital Heidelberg)
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IHC of human skin (courtesy of J.Heß, University Hospital Heidelberg)
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IHC of human skin (courtesy of J.Heß, University Hospital Heidelberg)
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WB with anti-HDC antibody (Cat. No. 16045, 1:2,500), RAW 264.7 whole cell lysate (3-10 µg)
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IHC of human skin (courtesy of J.Heß, University Hospital Heidelberg)
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WB with anti-HDC antibody (Cat. No. 16045, 1:5000) and secondary anti-rabbit HRP antibody (Cat. No. 90003, 1:50,000), sample Raw246.7 whole cell lysate (6.7 µg)
View more
IHC of human skin (courtesy of J.Heß, University Hospital Heidelberg)
View more
IHC of human skin (courtesy of J.Heß, University Hospital Heidelberg)
View more
IHC of human skin (courtesy of J.Heß, University Hospital Heidelberg)
View more
WB with anti-HDC antibody (Cat. No. 16045, 1:2,500), RAW 264.7 whole cell lysate (3-10 µg)
View more
IHC of human skin (courtesy of J.Heß, University Hospital Heidelberg)
View more
Key Features
- Rabbit polyclonal
- Suitable for ICC/IF, IHC on frozen and paraffin sections and WB
- Reacts with dog, guinea pig, human, mouse, rat
Product description
| Quantity | 50 µl (lyoph.) |
|---|---|
| Antibody Type | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Application | ICC/IF, IHC, WB |
| Purification | Undiluted antiserum |
| Reactivity | Dog, Guinea pig, Human, Mouse, Rat |
| Storage before reconstitution | 2-8°C until indicated expiry date |
| Storage after reconstitution | Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
| Intended use | Research use only |
| Immunogen | Recombinant histidine decarboxylase produced in E. coli |
| Formulation | Lyophilized; reconstitute in 100 µl dist. water |
| UniprotID | E2RMU1 (Dog, Canis familiaris),H0WB71 (Guinea pig),P19113 (Human),Q8CHP0 (Mouse),P16453 (Rat) |
| Synonym | Histidine decarboxylase, HDC, EC 4.1.1.22, HDC |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | Assay dependent |
| Immunohistochemistry (IHC) - frozen | 1:2,000-1:5,000 |
| Immunohistochemistry (IHC) - paraffin | 1:2,000-1:5,000 (microwave treatment recommended) |
| Western Blot (WB) | 1:1,000-1:5,000 |
Background
Histidine decarboxylase (HDC) is the enzyme catalyzing the conversion of histidine into histamine. HDC can be found in the histamine secreting ECL cells of some species as well as in the mast cells. Absorption with 10-100 ug immunogen per ml diluted antiserum abolishesthe staining.
In Western blot the antiserum detects the 54 kDa and 73 kDa forms in addition to a 63 kDa form (rat stomach, see Dartsch et al., 1998).
Positive control: Stefanini-fixed frozen sections of rat fundus.
Dartsch, C., Chen, D. & Persson, L. Multiple forms of rat stomach histidine decarboxylase may reflect posttranslational activation of the enzyme. Regul. Pept. 77, 33-41 (1998).
References/Publications (8)
Publication
Species
Application
Species
mouse
Application
IHC-IF
Species
mouse
Application
IHC-IF (frozen)
Species
human
Application
IHC (paraffin)
Species
rat
Application
IHC (paraffin)
Species
rat
Application
WB
Species
rat
Application
WB,IP
Species
rat
Application
ICC-IF
Species
rat
Application
IHC (frozen)
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FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
The expiration date of our antibodies is indicated on the product label.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet.
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.
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