anti-Human IgA Secretory Component mouse monoclonal, SC-05, purified

Cat. No. 691720

Available, delivery time 1-7 days

€340.00*

Key Features
  • Mouse monoclonal
  • Suitable for ELISA, FACS, IHC, IP and WB
  • Reacts with human and rat
  • Isotype: IgG1 kappa
Product description
Quantity 1 ml (100 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG1 kappa
Conjugate Unconjugated
Application ELISA, FACS, IHC, IP, WB
Purification Affinity chromatography
Reactivity Human, Rat
Storage 2-8°C
Intended use Research use only
Clone SC-05
Immunogen Affinity purified human secretory component
Concentration 100 µg/ml
Formulation PBS with 0.02% sodium azide
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:50-1:100 (1-2 µg/ml)
Immunohistochemistry (IHC) - paraffin 1:50-1:100 (1-2 µg/ml; microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
ELISA Assay dependent
Flow Cytometry (FACS) 0.5-1.0 µg/million cells in 0.1 ml
Immunoprecipitation (IP) Assay dependent
Western Blot (WB) 1:50-1:100 (1-2 µg/ml)
Background

SC-05 reacts with a reduction resistant epitope on 80 kDa human secretory component (both free and bound to SIgA). Secretory component is differentially expressed in epithelium, thus SC-05 can identify subpopulations of epithelial cells and epithelial differentiation. Secretory component negative cell lines are not stained with SC-05.

Positive control: Stomach, lung, or breast tumor.

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