anti-Insulin mouse monoclonal, 2D11-H5, purified

Cat. No. 691512

Available, delivery time 1-7 days

€340.00*

Key Features
  • Mouse monoclonal
  • Suitable for ELISA, FACS, ICC/IF and IHC
  • Reacts with bovine, human, mouse, pig and rat
  • Isotype: IgG1 kappa
Product description
Quantity 1 ml (100 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG1 kappa
Conjugate Unconjugated
Application ELISA, FACS, ICC/IF, IHC
Purification Affinity chromatography
Reactivity Bovine, Human, Mouse, Pig, Rat
Storage 2-8°C
Intended use Research use only
Clone 2D11-H5
Immunogen Purified pig insulin, conjugated to KLH
Concentration 100 µg/ml
Formulation PBS with 0.02% sodium azide
UniprotID P01317 (Bovine), P01308 (Human), P01326 (Mouse), P01315 (Pig), P01323 (Rat)
Synonym Insulin [Cleaved into: Insulin B chain; Insulin A chain], INS
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:100-1:200 (0.5-1.0 µg/ml)
Immunohistochemistry (IHC) - frozen 1:50-1:100 (1-2 µg/ml)
Immunohistochemistry (IHC) - paraffin 1:50-1:100 (1-2 µg/ml; microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
ELISA Assay dependent
Flow Cytometry (FACS) 0.5-1.0 µg/million cells in 0.1 ml
Background

Insulin is a protein consisting of an alpha-chain of 21 amino acids and a beta-chain of 30 amino acids and produced in the beta-cells of the pancreas. 2D11-H5 is a specific insulin antibody as tested by ELISA and on human pancreatic tissue.

Positive control: MIA, PaCa-2 cells, pancreas or insulinoma.

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