anti-Keratin 13+10 mouse monoclonal, EBS-IF-003, purified

Cat. No. 691657

Available, delivery time 1-7 days

€340.00*

Key Features
  • Mouse monoclonal
  • Suitable for FACS, ICC/IF, IHC and WB
  • Reacts with cat, dog and human
  • Isotype: IgG2a kappa
Product description
Quantity 1 ml (100 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG2a kappa
Conjugate Unconjugated
Application FACS, ICC/IF, IHC, WB
Purification Affinity chromatography
Reactivity Cat, Dog, Human
Storage 2-8°C
Intended use Research use only
Clone EBS-IF-003
Immunogen Cytoskeleton preparation from HeLa cells
Concentration 100 µg/ml
Formulation PBS with 0.02% sodium azide
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:50-1:100 (1-2 µg/ml)
Immunohistochemistry (IHC) - frozen 1:25-1:50 (2-4 µg/ml)
Immunohistochemistry (IHC) - paraffin 1:25-1:50 (2-4 µg/ml; microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
Flow Cytometry (FACS) 1-2 µg/million cells in 0.1 ml, fix cells in 4% PFA for 10 min at 4°C, permeabilize with 0.2% saponin or digitonon vor 15 min at 4°C
Western Blot (WB) 1:50-1:100 (1-2 µg/ml)
Background

EBS-IF-003 reacts with 53 kDa (CK13) and 56.6 kDa (CK10) cytokeratin proteins as indicated by immunoblotting. On formalin-fixed, paraffin-embedded tissue sections EBS-IF-003 recognizes only CK13. On cryostat sections EBS-IF-003 serves as differentiation-related marker of all stratified epithelia; it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas. In paraffin sections EBS-IF-003 does not stain CK10 positive, CK13 negative epithelia, as for example epidermis.

Positive control: A431, HeLa, MCF7 cells. Tonsil, esophageal or bladder carcinoma.

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