anti-Keratin 4, 5, 6, 8, 10, 13, 18 mouse monoclonal, C-11, purified

Cat. No. 691544

Available, delivery time 1-7 days

€340.00*

Key Features
  • Mouse monoclonal
  • Suitable for FACS, ICC/IF, IHC and WB
  • Reacts with cat, chicken, dog, ferret, goat, guinea pig, human, monkey, mouse, rabbit, rat and xenopus
  • Isotype: IgG1 kappa
Product description
Quantity 1 ml (100 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG1 kappa
Conjugate Unconjugated
Application FACS, ICC/IF, IHC, WB
Purification Affinity chromatography
Reactivity Cat, Chicken, Dog, Ferret, Goat, Guinea pig, Human, Monkey, Mouse, Rabbit, Rat, Xenopus
Storage 2-8°C
Intended use Research use only
Clone C-11
Immunogen Cytoskeleton preparation of human A431 carcinoma cell line
Concentration 100 µg/ml
Formulation PBS with 0.02% sodium azide
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:100-1:200 (0.5-1.0 µg/ml)
Immunohistochemistry (IHC) - frozen 1:50-1:100 (1-2 µg/ml)
Immunohistochemistry (IHC) - paraffin 1:50-1:100 (1-2 µg/ml; microwave treatment in 10 mM citrate buffer pH 6.0 or digestion with trypsin at 1 mg/ml PBS recommended)
Flow Cytometry (FACS) 0.5-1.0 µg/million cells in 0.1 ml, fix cells in 4% PFA for 10 min at 4°C, permeabilize with 0.2% saponin or digitonin for 15 min at 4°C
Western Blot (WB) 1:100 (1 µg/ml)
Background

C11 reacts with keratins: 4, 5, 6, 8, 10, 13 and 18. This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Positive control: A431 cells, skin, lung.

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