Species/Reactant: Homo sapiens (Human)
Applications: Immunohistochemistry-immunofluorescence
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
Species/Reactant: Homo sapiens (Human)
Applications: Immunohistochemistry-immunofluorescence
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
- Hybridoma cell culture supernatant
- Mouse monoclonal
- Suitable for IHC on frozen and paraffin sections and WB
- Reacts with human
- Isotype: IgG1
Product description
| Quantity | 1 ml |
|---|---|
| Antibody Type | Monoclonal |
| Host | Mouse |
| Isotype | IgG1 |
| Conjugate | Unconjugated |
| Application | IHC, WB |
| Purification | Hybridoma cell culture supernatant |
| Reactivity | Human |
| Storage | Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
| Intended use | Research use only |
| Clone | DE-K10 |
| Immunogen | Cytoskeletal preparation from human epidermis |
| Formulation | Contains 0.09% sodium azide |
| UniprotID | P13645 (Human) |
| Synonym | Keratin, type I cytoskeletal 10, Cytokeratin-10, CK-10, Keratin-10, K10, KRT10, KPP |
| Note | Centrifuge prior to opening |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunohistochemistry (IHC) - frozen | 1:5-1:20 |
| Immunohistochemistry (IHC) - paraffin | 1:5-1:20 (protease treatment recommended) |
| Western Blot (WB) | 1:100-1:250 |
Background
Mouse monoclonal antibody DE-K10 specifically recognizes the 56.6 kDa keratin K10 (formerly also designated cytokeratin 10) polypeptide in squamous cells and carcinomas from e.g. epidermis, lung, bladder, cervix, oesophagus.
Positive control: squamous cell carcinoma.
References/Publications (9)
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FAQs
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
Positive tissue: outer root sheath of hair follicle, sweat gland epithelium on the foot pads, filiform papillae of tongue.
- Homogenization (e.g. with polytron) of tissue samples in buffer L (140 mM NaCl, 5 mM EDTA, 5 mM EGTA, 10 mM Tris-HCl pH7.6) supplemented with protease inhibitors; use 1 ml buffer for approx. 0.1 g tissue.
- To reduce viscosity by high DNA contents, benzonase treatment can be included (30 min at 37°C).
- Centrifugation for 10 min (max. speed, table top centrifuge).
- Resuspend pellet in "Triton extraction buffer" (buffer L plus 1% Triton X-100) using e.g. a dounce homogenizer.
- Centrifugation for 10 min (max. speed, table top centrifuge).
- Resuspend pellet in "high salt extraction buffer" (buffer L plus 1.5 M KCl) using e.g. a dounce homogenizer.
- Centrifugation for 10 min (max. speed, table top centrifuge).
- Resuspend pellet in "Triton extraction buffer" (buffer L plus 1% Triton X-100) using e.g. a dounce homogenizer.
- Centrifugation for 10 min (max. speed, table top centrifuge).
- Resuspend pellet in Laemmli buffer and boil (5 min) for SDS-PAGE.
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