anti-Keratin K13 mouse monoclonal, Ks13.1, prediluted, purified
- Purified, prediluted
- Mouse monoclonal
- Suitable for ICC/IF, IHC and WB
- Reacts with bovine, human, rat
- Isotype: IgG1
|Application||ICC/IF, IHC, WB|
|Reactivity||Bovine, Human, Rat|
|Storage||Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles|
|Intended use||Research use only|
|Immunogen||Keratin K13 of Mr 54,000 purified from human esophagus|
|Formulation||PBS with 0.5% BSA and 0.09% sodium azide|
|Tested applications||Tested dilutions|
|Immunocytochemistry (ICC)/ Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (IHC) - frozen||1:50|
|Immunohistochemistry (IHC) - paraffin||1:50 (microwave treatment recommended)|
|Western Blot (WB)||Assay dependent|
Ks 13.1 represents an excellent marker to discriminate non-cornified squamous epithelia from those of different origin.
Polypeptide reacting: Mr 54,000 polypeptide human keratin K13 (formerly designated cytokeratin 13; with minor affinity to keratins K14, Mr 50,000, and K16, Mr 48,000 and also to K25, formerly designated K25irs1 from inner root sheath of hair follicle).
Tumors specifically detected: several squamous cell carcinomas, e.g. cervix carcinoma; transitional cell carcinoma of the bladder; craniopharyngioma.
Reactivity on cultured cell lines: cell lines from squamous cell CA, e.g. A-431 from epidermoid CA of vulva; RT 112, RT-4 of urinary bladder CA.
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