Disease related
Frequently used

anti-Keratin K18 mouse monoclonal, Ks18.04, prediluted, purified

Liquid

Liquid

Lyophilized

Prediluted

Sample

FITC

Biotin

Cat. No. 65028

Available, delivery time 1-7 days

€146.00*

Key Features
  • Purified, prediluted
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with bovine, dog, hamster, human, mouse, pig, rat, sheep, trout, zebrafish
  • Isotype: IgG1
Product description
Quantity 5 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application ICC/IF, IHC, WB
Purification Affinity chromatography
Reactivity Bovine, Dog, Hamster, Human, Mouse, Pig, Rat, Sheep, Trout, Zebrafish
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone Ks18.04
Immunogen Human keratin K18 from HeLa cytoskeletal preparation
Formulation PBS pH 7.4 with 0.5% BSA and 0.09% sodium azide
UniprotID A1XEA5 (Bovine),E2REU6 (Dog, Canis familiaris),P05783 (Human),P05784 (Mouse),F1SGG1 (Pig),H0UYZ2 (Guinea pig),Q5BJY9 (Rat),W5Q5M3 (Sheep)
Synonym Keratin, type I cytoskeletal 18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, CK-18, Keratin-18, K18, KRT18, CYK18, PIG46
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunohistochemistry (IHC) - frozen Ready-to-use
Immunohistochemistry (IHC) - paraffin Ready-to-use (microwave treatment recommended)
Western Blot (WB) 1:10-1:50
Background

Ks18.04 represents an excellent marker to discriminate simple epithelia from those of different origin. Tumors specifically detected: all adenocarcinoma; mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma. Polypeptide reacting: Mr 45,000 polypeptide (human keratin K18; formerly also designated cytokeratin 18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia.

Tested cultured cell lines: MCF-7.

References/Publications (12)
Publication
Species
Application
Downloads
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Category
Size
Filetype
Category Protocol
Size 924.86 KB
Filetype pdf
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