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anti-Keratin K19 mouse monoclonal, Ks19.1 (A53-B/A2), liquid, purified

IHC analysis of human kidney using anti-Keratin K19 antibody. IHC was performed on formalin fixed paraffin embedded sections. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. After preparation the tissue was blocked with normal serum for 20 min at RT. The primary antibody anti-Keratin K19 was diluted in PBS (antibody concentration 2 ug/ml) and incubated at 4°C over-night. The secondary antibody ImmPRESS HRP anti-mouse IgG was incubated for 20 min at RT. Slides were incubated with DAB solution until a brown staining is visable and with Haemalaun for a few minutes. The 20x picture was acquired using microscopy (courtesy of J. Hess, University Hospital Heidelberg).
IHC analysis of human head and neck squamous cell carcinoma using anti-Keratin K19 antibody (Cat. No. 690010). IHC was performed on formalin fixed paraffin embedded sections. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. After preparation the tissue was blocked with normal serum for 20 min at RT. The primary antibody anti-Keratin K19 (Cat. No. 690010) was diluted in PBS (antibody concentration 0.1 ug/ml) and incubated at 4°C over-night. The secondary antibody ImmPRESS HRP anti-mouse IgG was incubated for 20 min at RT. Slides were incubated with DAB solution until a brown staining is visable and with Haemalaun for a few minutes. The 20x picture was acquired using microscopy (courtesy of J. Hess, University Hospital Heidelberg).
Western blot analysis of MCF-7 lysate with anti-Keratin K19 antibody (Cat. No. 61010). Western blot analysis was performed on 5 ug/10 ug MCF-7 lysate. Cells were lysed with RIPA buffer. The PVDF membrane was blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-Keratin K19 mouse monoclonal (Cat. No. 61010) was diluted in blocking buffer (antibody concentration 0.5 ug/ml) and incubated for 1 h at RT. The secondary antibody anti-mouse IgG goat polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 ug/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using PierceTM ECL Western Blotting Substrate.
IHC analysis of human liver using anti-Keratin K19 antibody (Cat. No. 61010). IHC was performed on formalin fixed paraffin embedded sections. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. After preparation the tissue was blocked with normal serum for 20 min at RT. The primary antibody anti-Keratin K19 (Cat. No. 61010) was diluted in PBS (antibody concentration 1 ug/ml) and incubated at 4°C over-night. The secondary antibody ImmPRESS HRP anti-mouse IgG was incubated for 20 min at RT. Slides were incubated with DAB solution until a brown staining is visable and with Haemalaun for a few minutes. The picture was acquired using microscopy (courtesy of J. Hess, University Hospital Heidelberg).
IHC analysis of human colon using anti-Keratin K19 antibody (Cat. No. 61010). IHC was performed on formalin fixed paraffin embedded sections. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. After preparation the tissue was blocked with normal serum for 20 min at RT. The primary antibody anti-Keratin K19 (Cat. No. 61010) was diluted in PBS (antibody concentration 10 ug/ml) and incubated at 4°C over-night. The secondary antibody ImmPRESS HRP anti-mouse IgG was incubated for 20 min at RT. Slides were incubated with DAB solution until a brown staining is visable and with Haemalaun for a few minutes. The picture was acquired using microscopy (courtesy of J. Hess, University Hospital Heidelberg).

Liquid

Liquid

Lyophilized

Prediluted

Cat. No. 690010

Prices excl. VAT

Available, delivery time: 1-7 days

€207.00*

Datasheet
Key Features
  • Purified, liquid
  • Mouse monoclonal
  • Suitable for ELISA, ICC/IF, IHC and WB
  • Reacts with human
  • Isotype: IgG2a
Product description
Quantity 1 ml (50 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG2a
Conjugate Unconjugated
Application ELISA, ICC/IF, IHC, WB
Purification Affinity chromatography
Reactivity Human
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone Ks19.1 (A53-B/A2)
Immunogen Keratin K19 of Mr 40 000; from cultured human MCF-7 cells
Concentration 50 µg/ml
Formulation Contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4
UniprotID P08727 (Human)
Synonym Keratin, type I cytoskeletal 19, Cytokeratin-19, CK-19, Keratin-19, K19, KRT19
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunohistochemistry (IHC) - frozen 1:5-1:50
Immunohistochemistry (IHC) - paraffin 1:5-1:50 (microwave treatment recommended)
ELISA Assay dependent
Western Blot (WB) 1:50-1:500
Background

Ks 19.1 represents an excellent marker to discriminate glandular epithelial carcinoma from those of different origin. No reaction with hepatocellular carcinoma. Polypeptide reacting: Mr 40,000 polypeptide (keratin K19; formerly also designated cytokeratin 19) of human glandular epithelia. The epitope has been localized on aa. 311-335 (QSQLSMKAALEDTLAETEARFGAQL) of the alpha-helical fragment.

Tumors specifically detected: all tested adenocarcinoma; cholangio carcinoma of liver; renal cell carcinoma; transitional cell carcinoma of the bladder; ovary carcinoma; squamous cell carcinoma of cervix, bronchus and lung (intermediate type); mesothelioma; carcinoid tumor of bronchus; breast carcinoma; thymoma.

Reactivity on cultured cell lines: MCF-7.

References/Publications (20)
Publication
Species
Application
Publication Mariani, R., Paranjpe,S., Dobrowolski, R. & Weber, G. 14-3-3 targets keratin intermediate filaments to mechanically sensitive cell-cell contacts. Mol Biol Cell. 31, 930-943(2020).
Species Xenopus
Application IHC-frozen/IF
Publication Line Hamelin, C. et al. Identification and verification of heat shock protein 60 as a potential serum marker for colorectal cancer. FEBS J. 278, 4845–4859 (2011).
Species human
Application WB
Publication Langbein, L., Yoshida, H., Praetzel-Wunder, S., Parry, D. A. & Schweizer, J. The Keratins of the Human Beard Hair Medulla: The Riddle in the Middle. J. Invest. Dermatol. 130, 55–73 (2010).
Species human
Application IHC (frozen)
Publication Höchtlen-Vollmar, W. et al. Occult epithelial tumor cells detected in bone marrow by an enzyme immunoassay specific for cytokeratin 19. Int. J. cancer 70, 396–400 (1997).
Species human
Application ELISA
Publication Dittadi, R. et al. Standardization of assay for cytokeratin-related tumor marker CYFRA21.1 in urine samples. Clin. Chem. 42, 1634–8 (1996).
Species human
Application ELISA
Publication Demirkesen, C., Hoede, N. & Moll, R. Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual cytokeratins. J. Cutan. Pathol. 22, 518–35 (1995).
Species human
Application IHC (paraffin)
Publication Sugama, Y. et al. Clinical usefulness of CYFRA assay in diagnosing lung cancer: measurement of serum cytokeratin fragment. Jpn. J. Cancer Res. 85, 1178–84 (1994).
Species human
Application ELISA
Publication Grenier, J. et al. Cyfra 21-1, a new marker of lung cancer. Nucl. Med. Biol. 21, 471–476 (1994).
Species human
Application ELISA
Publication Van Der Gaast ’, A. et al. Evaluation of a new tumour marker in patients with non-small-cell lung cancer: Cyfra 21.1. Br. J. Cancer 69, 525–528 (1994).
Species human
Application ELISA
Publication Stieber, P. et al. CYFRA 21-1. A new marker in lung cancer. Cancer 72, 707–13 (1993).
Species human
Application ELISA
Publication Pujol, J. L. et al. Serum fragment of cytokeratin subunit 19 measured by CYFRA 21-1 immunoradiometric assay as a marker of lung cancer. Cancer Res. 53, 61–6 (1993).
Species human
Application ELISA
Publication Lindberg, K. & Rheinwaldtt, J. G. Suprabasal 40 kd Keratin (K19) Expression as an Immunohistologic Marker of Premalignancy in Oral Epithelium. Am. J. Pathol. 134, 89–98 (1989).
Species human
Application IHC (paraffin)
Publication Moll, R. et al. Cytokeratins in normal and malignant transitional epithelium: maintenance of expression of urothelial differentiation features in transitional cell carcinomas and bladder carcinoma cell culture lines. Am. J. Pathol. 132, 123–144 (1988).
Species human
Application IHC (frozen),ICC-IF
Publication Bosch, F. X., Leube, R., Achtstätter, T., Moll, R. & Franke, W. W. Expression of Simple Epithelial Type Cytokeratins In Stratified Epithelia as Detected by Immunolocalization and Hybridization In Situ. J. Cell Biol. 106, 1635–1648 (1988).
Species human
Application IHC (frozen)
Publication Balaton, A. J., Nehama-Sibony, M., Gotheil, C., Callard, P. & Baviera, E. E. Distinction between hepatocellular carcinoma, cholangiocarcinoma, and metastatic carcinoma based on immunohistochemical staining for carcinoembryonic antigen and for cytokeratin
Species human
Application IHC (paraffin)
Publication Heid, H. W., Moll, I. & Franke, W. W. Patterns of expression of trichocytic and epithelial cytokeratins in mammalian tissues. I. Human and bovine hair follicles. Differentiation. 37, 137–57 (1988).
Species human
Application IHC (frozen)
Publication Moll, I; Heid, H; Moll, R. Cytokeratin analysis of pilomatrixoma: changes in cytokeratin-type expression during differentiation. J. Invest. Dermatol. 91, 251–7 (1988).
Species human
Application IHC (frozen)
Publication Kasper, M., Stosiek, P., Typlt, H. & Karsten, U. c. 1. Normal tissues. Eur. J. Cancer Clin. Oncol. 23, 137–47 (1987).
Species human
Application IHC (frozen)
Publication Dockhorn-Dworniczak, b. et al. patterns of expression of cytoskeletal proteins in human thyroid gland and thyroid carcinomas. differentiation. 35, 53–71 (1987).
Species human
Application IHC (frozen)
Publication Karsten, U. et al. Monoclonal anti-cytokeratin antibody from a hybridoma clone generated by electrofusion. Eur. J. Cancer Clin. Oncol. 21, 733–40 (1985).
Species human
Application ICC-IF
Downloads
File
Category
Size
Filetype
File IHC mouse antibody
Category Protocol
Size 167.19 KB
Filetype pdf
File WB protocol
Category Protocol
Size 210.69 KB
Filetype pdf
Datasheet
MSDS
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FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Please find the solvent for reconstitution and the amount of liquid for reconstitution on the antibody datasheet. The buffer formulation after reconstitution is also mentioned in the datasheet.
PROGEN offers different formats for mouse monoclonal antibodies.  Different antibodies may not be offered in each format, the following are all possible formats:
  1. Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
  2. Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet. 
  3. Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet. 
  4. Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.
We offer bulk amounts starting from 1 mg for many of our mouse monoclonal or recombinant antibodies. Please contact us for further information or use the “bulk button” on the product page.
Isolation of positive area from tissue slices under the microscope, followed by extraction procedure to enrich for the cytoskeleton.
Positive tissue: outer root sheath of hair follicle, sweat gland epithelium on the foot pads, filiform papillae of tongue.
  • Homogenization (e.g. with polytron) of tissue samples in buffer L (140 mM NaCl, 5 mM EDTA, 5 mM EGTA, 10 mM Tris-HCl pH7.6) supplemented with protease inhibitors; use 1 ml buffer for approx. 0.1 g tissue.
  • To reduce viscosity by high DNA contents, benzonase treatment can be included (30 min at 37°C).
  • Centrifugation for 10 min (max. speed, table top centrifuge).
  • Resuspend pellet in "Triton extraction buffer" (buffer L plus 1% Triton X-100) using e.g. a dounce homogenizer.
  • Centrifugation for 10 min (max. speed, table top centrifuge).
  • Resuspend pellet in "high salt extraction buffer" (buffer L plus 1.5 M KCl) using e.g. a dounce homogenizer.
  • Centrifugation for 10 min (max. speed, table top centrifuge).
  • Resuspend pellet in "Triton extraction buffer" (buffer L plus 1% Triton X-100) using e.g. a dounce homogenizer.
  • Centrifugation for 10 min (max. speed, table top centrifuge).
  • Resuspend pellet in Laemmli buffer and boil (5 min) for SDS-PAGE.

Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.

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Keratin

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