Diagnostic marker

anti-Keratin K20 mouse monoclonal, IT-Ks20.5, lyophilized, purified

Cat. No. 61033

Available, delivery time 1-7 days

€300.00*

Key Features
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with human and pig
  • Isotype: IgG2a
Product description
Quantity 50 µg
Antibody Type Monoclonal
Host Mouse
Isotype IgG2a
Conjugate Unconjugated
Application ICC/IF, IHC, WB
Purification Affinity chromatography
Reactivity Human, Pig
No reactivity Rat
Storage before reconstitution 2-8°C until indicated expiry date
Storage after reconstitution Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone IT-Ks20.5
Immunogen Electrophoretically purified keratin K20 from human intestinal mucosa
Concentration 50 µg/ml after reconstitution with 1ml dist. water
Formulation Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
UniprotID P35900 (Human), Q29218 (Pig)
Synonym Keratin, type I cytoskeletal 20, Cytokeratin-20, CK-20, Keratin-20, K20, Protein IT, KRT20
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunohistochemistry (IHC) - frozen Assay dependent
Western Blot (WB) Assay dependent
Background

IT-Ks 20.5 represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative.

Polypeptide recognized: protein IT (keratin K20; Mr 46,000; formerly also designated cytokeratin 20).

Reactivity on cultured cell lines: HT-29, LoVo, DLD-1, SW 1116, CaCo-2, RT-4.

References/Publications (2)
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