anti-Keratin K4 guinea pig polyclonal, serum

Cat. No. GP-K4

Available, delivery time 1-7 days

€256.00*

Key Features
  • Guinea pig polyclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with human
Product description
Quantity 100 µl
Antibody Type Polyclonal
Host Guinea pig
Conjugate Unconjugated
Application ICC/IF, IHC, WB
Purification Stabilized antiserum
Reactivity Human
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Immunogen Synthetic peptide (C-KIISTTTL NKRR) of human keratin K4 coupled to KLH
Formulation Contains 0.09% sodium azide and 0.5% BSA
UniprotID P19013 (Human)
Synonym Keratin, type II cytoskeletal 4, Cytokeratin-4, CK-4, Keratin-4, K4, Type-II keratin Kb4, KRT4, CYK4
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunohistochemistry (IHC) - frozen 1:200
Immunohistochemistry (IHC) - paraffin 1:50-1:200 (microwave treatment recommended)
Western Blot (WB) 1:3,000
Background

Excellent marker to discriminate non-cornified squamous epithelia (esophagus, vagina, gingival) from those of different origin. Recognizes keratin K4 in non-cornifying squamous epithelia.
Keratin K4 is mostly present in combination with keratin K13. In contrast to keratin K13, the superficial cells of the cornea are reactive with keratin K4. The antibody also stains ciliated pseudo-stratified epithelium of bronchi and, focally, ductal epithelial cells.

Reactive polypeptide: human type II (basic) keratin K4 (Mr 59,000), formerly also designated cytokeratin 4.

Positive control: squamous epithelium of esophagus, gingiva, palate, vagina (staining all suprabasal layers).

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