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anti-Keratin K4 mouse monoclonal, 6B10, supernatant

Cat. No. 10525

Prices excl. VAT

Available, delivery time: 1-7 days

€235.00*

Datasheet
Key Features
  • Mouse monoclonal
  • Suitable for IHC on frozen sections
  • Reacts with human
  • Isotype: IgG1
Product description
Quantity 1 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application IHC
Purification Hybridoma cell culture supernatant
Reactivity Human
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone 6B10
Immunogen Human esophagus
Formulation Contains 0.1% sodium azide
UniprotID P19013 (Human)
Synonym Keratin, type II cytoskeletal 4, Cytokeratin-4, CK-4, Keratin-4, K4, Type-II keratin Kb4, KRT4, CYK4
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:5-1:10
Background

6B10 recognizes keratin K4 in non-cornifying squamous epithelia. Keratin K4 is mostly present in combination with keratin K13. In contrast to keratin K13, the superficial cells of the cornea are reactive with keratin K4. 6B10 also stains ciliated pseudo-stratified epithelium of bronchi and, focally, ductal epithelial cells. Polypeptide reacting: 59 kDa keratin K4 (formerly also designated cytokeratin 4).

Positive control: squamous cell carcinomas.

References/Publications (10)
Publication
Species
Application
Publication Langbein, L. et al. New facets of keratin K77: Interspecies variations of expression and different intracellular location in embryonic and adult skin of humans and mice. Cell Tissue Res. 354, 793–812 (2013).
Species human
Application IHC (frozen)
Publication Corver, W. E. et al. Four-color multiparameter DNA flow cytometric method to study phenotypic intratumor heterogeneity in cervical cancer. Cytometry 39, 96–107 (2000).
Species human
Application FACS
Publication Schaafsma, H. E. & Ramaekers, F. C. Cytokeratin subtyping in normal and neoplastic epithelium: basic principles and diagnostic applications. Pathol. Annu. 29 Pt 1, 21–62 (1994).
Species human
Application IHC (frozen)
Publication Moll, R. et al. The human gene encoding cytokeratin 20 and its expression during fetal development and in gastrointestinal carcinomas. Differentiation. 53, 75–93 (1993).
Species human
Application IHC (frozen)
Publication Smedts, F. et al. Keratin expression in cervical cancer. Am. J. Pathol. 141, 497–511 (1992).
Species human
Application IHC (frozen)
Publication Smedts, F. et al. Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia. Am. J. Pathol. 136, 657–68 (1990).
Species human
Application IHC (frozen)
Publication Bosch, F. X. et al. Extensive changes in cytokeratin expression patterns in pathologically affected human gingiva. Virchows Arch. B. Cell Pathol. Incl. Mol. Pathol. 58, 59–77 (1989).
Species human
Application IHC (frozen)
Publication Leube, R. et al. Molecular Characterization and Expression of the Stratification-related Cytokeratins 4 and 15 Isolation of cDNA Clones Encoding. J. Cell Biol. 106, 1249–1261 (1988).
Species human
Application IHC (frozen)
Publication Moll, R. et al. Cytokeratins in normal and malignant transitional epithelium: maintenance of expression of urothelial differentiation features in transitional cell carcinomas and bladder carcinoma cell culture lines. Am. J. Pathol. 132, 123–144 (1988).
Species human
Application IHC (frozen),ICC-IF
Publication Van Muijen, G. N., Warnaar, S. O. & Ponec, M. Differentiation-related changes of cytokeratin expression in cultured keratinocytes and in fetal, ne328orn, and adult epidermis. Exp. Cell Res. 171, 331–45 (1987).
Species human
Application WB,IHC (frozen),ICC-IF
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Datasheet
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FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.

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