anti-Keratin K5/K8 (Pan Epithelial) mouse monoclonal, RCK102, supernatant

Cat. No. 10521

Available, delivery time 1-7 days

€239.00*

Key Features
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC on frozen and paraffin sections and WB
  • Reacts with dog, hamster, human, mouse, pig, rabbit and rat
  • Isotype: IgG1
Product description
Quantity 1 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application ICC/IF, IHC, WB
Purification Hybridoma cell culture supernatant
Reactivity Dog, Hamster, Human, Mouse, Pig, Rabbit, Rat
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone RCK102
Immunogen Lung cancer cell preparation
Formulation Contains 0.09% sodium azide
UniprotID Q7RTS7 (Human),Q922U2 (Mouse),A0A287BA49 (Pig),A0A286X8W5 (Guinea pig),A0A5F9C3E1 (Rabbit),Q6P6Q2 (Rat),Q6P6Q2 (Rat),P05787 (Human),P11679 (Mouse)
Synonym Keratin, type II cytoskeletal 74, Cytokeratin-74, CK-74, Keratin-5c, K5C, Keratin-74, K74, Type II inner root sheath-specific keratin-K6irs4, Type-II keratin Kb37, KRT74, K6IRS4, KB37, KRT5C, KRT6IRS4, Keratin, type II cytoskeletal 8, Cytokeratin-8, CK-8, Keratin-8, K8, Type-II keratin Kb8, KRT8, CYK8
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunohistochemistry (IHC) - frozen 1:5-1:10
Immunohistochemistry (IHC) - paraffin 1:5-1:10 (microwave treatment recommended)
Western Blot (WB) Assay dependent
Background

Mab RCK 102, directed against the 58 kD and 52.2 kD keratin proteins (keratins K5 and K8; formerly also designated cytokeratins 5 and 8 in the Moll catalogue of keratins), reacts with all epithelial cell types. No reaction is observed in non-epithelial tissues. The antibody can be used for the discrimination of carcinomas from non-epithelial tumors such as lymphomas, sarcomas and neural tumors. RCK 102 is applicable on frozen and paraffin-embedded tissue. Formalin fixation may cause antigen denaturation or masking. Therefore, protease digestion is necessary prior to antibody application.

Positive control: Skin, cervix.

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