anti-Keratin K7 guinea pig polyclonal, serum

Cat. No. GP-K7

Available, delivery time 1-7 days

€256.00*

Key Features
  • Guinea pig polyclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with human
Product description
Quantity 100 µl
Antibody Type Polyclonal
Host Guinea pig
Conjugate Unconjugated
Application ICC/IF, IHC, WB
Purification Stabilized antiserum
Reactivity Human
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Immunogen Synthetic peptide (C-SAGPGLLKAYSIRT) of human keratin K7 (formerly also designated cytokeratin 7), coupled to KLH
Formulation Contains 0.09% sodium azide and 0.5% BSA
UniprotID P08729 (Human)
Synonym Keratin, type II cytoskeletal 7, Cytokeratin-7, CK-7, Keratin-7, K7, Sarcolectin, Type-II keratin Kb7, KRT7, SCL
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunohistochemistry (IHC) - frozen 1:200
Immunohistochemistry (IHC) - paraffin 1:100 (microwave treatment recommended)
Western Blot (WB) 1:3,000
Background

Excellent marker for the discrimination of specific subtypes of adenocarcinoma: e.g. adenocarcinoma of pancreas, bile duct carcinoma and transitional carcinoma of bladder are stained, whereas hepatocellular and prostate carcinomas are negative.

Detects specific subtypes of adenocarcinomas: adenocarcinoma of pancreas, gallbladder, lung, cervix; cholangio carcinoma of liver; ductal and lobular carcinoma of breast; carcinomas of ovary; transitional cell carcinoma of bladder; mesothelioma; negative with most cases of hepatocellular carcinoma. In colorectal carcinoma early stages are reported to be negative, but advanced stages of tumor development are positive for keratin K7 expression.

Reactive polypeptide (specificity): basic human keratin K7 (Mr 54,000).

Positive control: glandular epithelia (e.g. sweat glands, sebaceous glands); Merkel cells.

References/Publications (1)
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