- Mouse monoclonal
- Suitable for ICC/IF and IHC on frozen and paraffin sections
- Reacts with human
- Isotype: IgG1
Product description
| Quantity | 1 ml |
|---|---|
| Antibody Type | Monoclonal |
| Host | Mouse |
| Isotype | IgG1 |
| Conjugate | Unconjugated |
| Application | ICC/IF, IHC |
| Purification | Hybridoma cell culture supernatant |
| Reactivity | Human |
| Storage | Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
| Intended use | Research use only |
| Clone | OVTL 12-30 |
| Immunogen | Cytoskeletal preparation from T24 cells |
| Formulation | Contains 0.09% sodium azide |
| UniprotID | P08729 (Human) |
| Synonym | Keratin, type II cytoskeletal 7, Cytokeratin-7, CK-7, Keratin-7, K7, Sarcolectin, Type-II keratin Kb7, KRT7, SCL |
| Note | Centrifuge prior to opening |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | Assay dependent |
| Immunohistochemistry (IHC) - frozen | 1:5-1:10 |
| Immunohistochemistry (IHC) - paraffin | 1:5-1:10 (microwave treatment recommended) |
Background
The monoclonal antibody OVTL 12-30 can be used to distinguish between ovarian carcinomas and colon carcinomas in routinely processed tissue sections. Immunoblotting : The antibody is reactive with the 54 kD cytokeratin protein (cytokeratin 7).
Positive control: Glandular epithelium breast
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FAQs
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
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