Species/Reactant: Homo sapiens (Human)
Applications: Western Blotting
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
Species/Reactant: Homo sapiens (Human)
Applications: Western Blotting
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
- Mouse monoclonal
- Suitable for ICC/IF, IHC on frozen and paraffin sections and WB
- Reacts with human
- Isotype: IgG1
Product description
| Quantity | 1 ml |
|---|---|
| Antibody Type | Monoclonal |
| Host | Mouse |
| Isotype | IgG1 |
| Conjugate | Unconjugated |
| Application | ICC/IF, IHC, WB |
| Purification | Hybridoma cell culture supernatant |
| Reactivity | Human |
| Storage | Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
| Intended use | Research use only |
| Clone | NCL-5D3 |
| Immunogen | Isolated from human breast cancer carcinoma cell line MCF-7 |
| Formulation | Contains 0.1% sodium azide |
| UniprotID | P05787 (Human) |
| Synonym | Keratin, type II cytoskeletal 8, Cytokeratin-8, CK-8, Keratin-8, K8, Type-II keratin Kb8, KRT8, CYK8 |
| Note | Centrifuge prior to opening |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | 1:5-1:10 |
| Immunohistochemistry (IHC) - frozen | 1:5-1:10 |
| Immunohistochemistry (IHC) - paraffin | 1:5-1:10 (protease treatment and/or microwave treatment recommended) |
| Western Blot (WB) | Assay dependent |
Background
The antibody recognizes all simple epithelia including glandular epithelia. It does not react with stratified squamous epithelium. Useful for the differentiation between carcinomas and lymphomas. In immunoblotting (Western) NCL 5D3 is directed against the the 52.2 kDa keratin K8 (originally reported also to 45 kDa keratin K18; formerly also designated cytokeratins according to the Moll-catalogue). Epitope has been mapped to aa 353-367 on alpha helical rod domain of keratin K8 (Waseem et al., 2004).
Positive control: glandular epithelium.
Waseem A, Karsten U, Leigh IM, Purkis P, Waseem NH, Lane BE: Conformational changes in the rod domain of human keratin 8 following heterotypic association with keratin 18 and its implication for filament stability. Biochemistry 43, 1283-1295 (2004).
References/Publications (2)
FAQs
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
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