anti-Keratin Pan guinea pig polyclonal + anti-guinea pig IgG HRP set

Cat. No. 71004

Available, delivery time 1-7 days

€327.00*

Key Features
The set provides primary anti-Keratin Pan guinea pig polyclonal antibody and secondary anti-guinea pig IgG HRP antibody for western blot.
The anti-Keratin Pan antibody covers all types of epithelia and epithelium-derived neoplasms.

Included products:

Product description
Quantity 100 µl anti-Keratin Pan + 100 µg anti-guinea pig IgG HRP
Antibody Type Polyclonal
Host Goat, Guinea pig
Conjugate Unconjugated
Application IHC, WB
Reactivity See individual antibody datasheet
Storage Lyophilized at 2-8°C; reconstituted at -20°C (avoid freeze/thaw cycles)
Intended use Research use only
Immunogen See individual antibody datasheet for information about specific immunogens
Formulation Keratin Pan antibody: contains 0.09% sodium azide and 0.5% BSA; anti-guinea pig IgG HRP antibody: lyophilized, reconstitute in 500 µl dist. water (final solution contains 10 mg/ml BSA in PBS buffer, pH 7.4)
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen anti-Keratin Pan: 1:100-1:200
Immunohistochemistry (IHC) - paraffin anti-Keratin Pan: 1:50-1:200 (microwave treatment recommended)
Western Blot (WB) anti-Keratin Pan: 1:1,000-1:2,000; anti-guinea pig HRP: 1:2,500-1:100,000
Background

anti-Keratin Pan antibody:
Reactive Polypeptides: Acidic (K1, K4, K8) and basic (K14) keratins (40 - 68 kDa polypeptides).
Tumors specifically detected: All types of epithelia and epithelium-derived neoplasms.

anti-guinea pig IgG goat polyclonal, HRP conjugate:
Whole Guinea Pig IgG secondary antibodies are isolated from antisera by immunoaffinity chromatography. The antibody is conjugated to Horseradish peroxidase (HRP) and is commonly used for WB, IHC and ELISA.
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule guinea pig IgG. It also reacts with the light chains of other guinea pig immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.

Set content:
Cat. No. GP14, anti-Keratin Pan guinea pig polyclonal, serum
Cat. No. 90001, anti-guinea pig IgG goat polyclonal, HRP conjugate

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