Epitope mapped
Disease related
Frequently used

anti-Keratin Type II mouse monoclonal, AE3, purified

Cat. No. 61806

Available, delivery time 1-7 days

€224.00*

Key Features
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with bovine, chicken, human, monkey, mouse, rabbit and rat
  • Isotype: IgG1
Product description
Quantity 1 ml (200 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application ICC/IF, IHC, WB
Purification Affinity chromatography
Reactivity Bovine, Chicken, Human, Monkey, Mouse, Rabbit, Rat
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone AE3
Immunogen Human epidermal keratin
Concentration 200 µg/ml
Formulation PBS pH 7.4 with 0.09% sodium azide
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunohistochemistry (IHC) - frozen 1:100
Immunohistochemistry (IHC) - paraffin 1:100 (protease treatment and/or microwave treatment recommended)
Western Blot (WB) Assay dependent
Background

AE 3 represents an excellent marker for distinguishing carcinoma from non-epithelial tumors. Tumors specifically detected: all epithelia and their neoplasms.
Polypeptide reacting: Mr 53,000 to Mr 67,000 basic type II keratin polypeptides of human epithelial proteins (keratins - formerly also designated cytokeratins - nos. K1 - K8). Epitope has been mapped to aa 266-299 on alpha-helical rod domain (see Waseem et al., 2004).
Reactivity on cultured cell lines: MCF-7, A-431, RT 112.


Waseem A, Karsten U, Leigh IM, Purkis P, Waseem NH, Lane BE: Conformational changes in the rod domain of human keratin 8 following heterotypic association with keratin 18 and its implication for filament stability. Biochemistry 43, 1283-1295 (2004)

References/Publications (10)
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