Disease related
Frequently used

anti-Keratin Type II mouse monoclonal, Ks pan1-8, lyophilized, purified

Cat. No. 61006

Available, delivery time 1-7 days

€207.00*

Key Features
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with amphibia, bovine, human, mouse and rat
  • Isotype: IgG2a
Product description
Quantity 50 µg
Antibody Type Monoclonal
Host Mouse
Isotype IgG2a
Conjugate Unconjugated
Application ICC/IF, IHC, WB
Purification Affinity chromatography
Reactivity Amphibia, Bovine, Human, Mouse, Rat
Storage before reconstitution 2-8°C until indicated expiry date
Storage after reconstitution Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone Ks pan1-8
Immunogen Cytoskeletal proteins from cultured human MCF-7 cells
Concentration 50 µg/ml after reconstitution with 1 ml dist. water
Formulation Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunohistochemistry (IHC) - frozen 1:10-1:100
Immunohistochemistry (IHC) - paraffin 1:10-1:100 (microwave treatment recommended)
Western Blot (WB) Assay dependent
Background

Ks pan1-8 represents an excellent marker for distinguishing carcinomas from non-epithelial tumors.
Polypeptides reacting: Mr 52,500-Mr 68,000 keratins (type II keratins K1-K8; formerly also designated cytokeratins 1-8) of human epithelial cells.
Tumors specifically detected: all epithelium-derived neoplasms.

Reactivity on cultured cell lines MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650, SSC-12.

References/Publications (6)
Publication
Species
Application
Downloads
File
Category
Size
Filetype
Category Protocol
Size 924.86 KB
Filetype pdf
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