Epitope mapped
Disease related
Frequently used

anti-LDL Receptor mouse monoclonal, IgG-C7, lyophilized, purified

Cat. No. 61087

Available, delivery time 1-7 days

€241.00*

Key Features
  • Mouse monoclonal
  • Suitable for FACS, IHC and ICC/IF
  • Reacts with bovine and human
  • Isotype: IgG2b kappa
Product description
Quantity 50 µg
Antibody Type Monoclonal
Host Mouse
Isotype IgG2b kappa
Conjugate Unconjugated
Application FACS, ICC/IF
Purification Affinity chromatography
Reactivity Bovine, Human
No reactivity Dog, Hamster, Mouse, Rabbit, Rat
Storage before reconstitution 2-8°C until indicated expiry date
Storage after reconstitution Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone IgG-C7
Immunogen Purified bovine adrenal cortex LDL receptor
Concentration 50 µg/ml after reconstitution with 1 ml dist. water
Formulation Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.5% BSA in PBS buffer, pH 7.4)
UniprotID P01131 (Bovine), P01130 (Human)
Synonym Low-density lipoprotein receptor, LDL receptor, LDLR
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:10–1:100
Immunohistochemistry (IHC) - paraffin 1:100
Flow Cytometry (FACS) Assay dependent
Background
Suitable for analysis of LDL receptor function in patients with familial hypercholesterolemia. The antibody recognizes an epitope in the region of repeat #1 of the ligand binding region. Addition of 15 nM antibody results in inhibition of half-maximal LDL-binding (Beisiegel et al. 1981). In human normal fibroblasts the antibody detects the 160 kDa polypeptide (pI 4.3) and also in bovine adrenal gland (160 kDa; pI 4.6) of LDL receptors (Beisiegel et al. 1982).

Beisiegelt, U., Schneider, W. J., Brown, M. S. & Goldstein, J. L. Immunoblot Analysis of Low Density Lipoprotein Receptors in Fibroblasts from Subjects with Familial Hypercholesterolemia. J. Biol. Chem. 257, 13150–13156 (1982).
Beisiegel, U., Schneider, W. J., Goldstein, J. L., Anderson, R. G. & Brown, M. S. Monoclonal antibodies to the low density lipoprotein receptor as probes for study of receptor-mediated endocytosis and the genetics of familial hypercholesterolemia. J. Biol. Chem. 256, 11923–31 (1981).

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