anti-LDL Receptor rabbit polyclonal, Biotin Conjugate
- Biotin conjugate
- Rabbit polyclonal
- Suitable for FACS and Receptor Binding Assays
- Reacts with human
|Application||FACS, Receptor binding assay|
|Intended use||Research use only|
|Immunogen||Specific synthetic peptide (sequence not conserved in VLDL receptor and LRP) of the human LDL receptor extracellular domain (epitope between residues nos. 184-195, the linker region between repeats 4 and 5)|
|Formulation||PBS pH 7.4 with penicillin/streptomycin|
|UniprotID||P01130 (Human), P01131 (Bovine), P35951 (Mouse), P35952 (Rat)|
|Synonym||Low-density lipoprotein receptor, LDL receptor, LDLR|
|Note||Centrifuge prior to opening|
|Tested applications||Tested dilutions|
|Flow Cytometry (FACS)||1:10|
|Receptor Binding Assays||Assay dependent|
The LDL receptor (160 kDa mature or glycosylated receptor; 120 kDa precursor or unglycosylated receptor) plays a key role in cellular cholesterol homeostasis.
The antibody to LDLR reacts specifically with human LDLR and is suitable for use in flow cytometry and immunoblotting. The antibody does not inhibit binding of LDL.
In immunoblotting, the antibody recognizes the 160 kDa band of LDLR and a 120 kDa band of the LDLR precursor from fibroblasts, hepatocytes, and monocytic cells cultured in the presence of lipoprotein-deficient serum.
Q & A's
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The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at firstname.lastname@example.org.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.