anti-LDL Receptor rabbit polyclonal, purified

Biotin

Serum

Cat. No. 61099

Available, delivery time 1-7 days

€251.00*

Key Features
  • Purified serum
  • Rabbit polyclonal
  • Suitable for FACS, IHC, Receptor Binding Assays and WB
  • Reacts with bovine, human, mouse and rat
Product description
Quantity 250 µl (100 µg/ml)
Antibody Type Polyclonal
Host Rabbit
Isotype IgG
Conjugate Unconjugated
Application FACS, IHC, Receptor binding assay, WB
Purification Affinity chromatography
Reactivity Bovine, Human, Mouse, Rat
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Immunogen Specific synthetic peptide (sequence not conserved in VLDL receptor and LRP) of the human LDL receptor extracellular domain (epitope between residues nos. 184-195, the linker region between repeats 4 and 5)
Concentration 100 µg/ml
Formulation PBS with 0.5% BSA
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:20-1:50
Immunohistochemistry (IHC) - paraffin 1:20-1:50 (microwave treatment recommended)
Flow Cytometry (FACS) 1:10
Receptor Binding Assays Assay dependent
Western Blot (WB) 1:100-1:200
Background

The LDL receptor (160 kDa mature or glycosylated receptor; 120 kDa precursor or unglycosylated receptor) plays a key role in cellular cholesterol homeostasis.
The antibody to LDLR reacts specifically with human LDLR and is suitable for use in flow cytometry and immunoblotting. The antibody does not inhibit binding of LDL.
In immunoblotting, the antibody recognizes the 160 kDa band of LDLR and a 120 kDa band of the LDLR precursor from fibroblasts, hepatocytes, and monocytic cells cultured in the presence of lipoprotein-deficient serum.

References/Publications (3)
Downloads
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Size
Filetype
Category Protocol
Size 925.08 KB
Filetype pdf
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