anti-LDL Receptor rabbit polyclonal, purified
Human liver (courtesy of J.Heß, University Hospital Heidelberg)
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Human liver (courtesy of J.Heß, University Hospital Heidelberg)
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Human liver (courtesy of J.Heß, University Hospital Heidelberg)
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Human liver (courtesy of J.Heß, University Hospital Heidelberg)
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WB with anti-LDL Receptor antibody (Cat. No. 61099, 1:100), A-172 whole cell lysate (3-10 µg)
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Jiang, L., Wu, W. F., et al. The use of targeted exome sequencing in genetic diagnosis of young patients with severe hypercholesterolemia. Sci Rep. 2016-11-10.
Species/Reactant: Homo sapiens (Human)
Applications: Western Blotting
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
View moreSpecies/Reactant: Homo sapiens (Human)
Applications: Western Blotting
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
Benito-Vicente, A., Siddiqi, H., et al. p.(Asp47Asn) and p.(Thr62Met): non deleterious LDL receptor missense variants functionally characterized in vitro. Sci Rep. 2018-11-09.
Species/Reactant: Cricetulus griseus (Chinese hamster)
Applications: Western Blotting
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
View moreSpecies/Reactant: Cricetulus griseus (Chinese hamster)
Applications: Western Blotting
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
Galicia-Garcia, U., Benito-Vicente, A., et al. Mutation type classification and pathogenicity assignment of sixteen missense variants located in the EGF-precursor homology domain of the LDLR. Sci Rep. 2020-02-03.
Species/Reactant: Cricetulus griseus (Chinese hamster)
Applications: Western Blotting
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
View moreSpecies/Reactant: Cricetulus griseus (Chinese hamster)
Applications: Western Blotting
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
Galicia-Garcia, U., Benito-Vicente, A., et al. Mutation type classification and pathogenicity assignment of sixteen missense variants located in the EGF-precursor homology domain of the LDLR. Sci Rep. 2020-02-03.
Species/Reactant: Cricetulus griseus (Chinese hamster)
Applications: Western Blotting
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
View moreSpecies/Reactant: Cricetulus griseus (Chinese hamster)
Applications: Western Blotting
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
Galicia-Garcia, U., Benito-Vicente, A., et al. Mutation type classification and pathogenicity assignment of sixteen missense variants located in the EGF-precursor homology domain of the LDLR. Sci Rep. 2020-02-03.
Species/Reactant: Cricetulus griseus (Chinese hamster)
Applications: Western Blotting
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
View moreSpecies/Reactant: Cricetulus griseus (Chinese hamster)
Applications: Western Blotting
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
Galicia-Garcia, U., Benito-Vicente, A., et al. Mutation type classification and pathogenicity assignment of sixteen missense variants located in the EGF-precursor homology domain of the LDLR. Sci Rep. 2020-02-03.
Species/Reactant: Cricetulus griseus (Chinese hamster)
Applications: Western Blotting
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
View moreSpecies/Reactant: Cricetulus griseus (Chinese hamster)
Applications: Western Blotting
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
Human liver (courtesy of J.Heß, University Hospital Heidelberg)
View more
Human liver (courtesy of J.Heß, University Hospital Heidelberg)
View more
Human liver (courtesy of J.Heß, University Hospital Heidelberg)
View more
Human liver (courtesy of J.Heß, University Hospital Heidelberg)
View more
WB with anti-LDL Receptor antibody (Cat. No. 61099, 1:100), A-172 whole cell lysate (3-10 µg)
View more
Jiang, L., Wu, W. F., et al. The use of targeted exome sequencing in genetic diagnosis of young patients with severe hypercholesterolemia. Sci Rep. 2016-11-10.
Species/Reactant: Homo sapiens (Human)
Applications: Western Blotting
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
View moreSpecies/Reactant: Homo sapiens (Human)
Applications: Western Blotting
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
Benito-Vicente, A., Siddiqi, H., et al. p.(Asp47Asn) and p.(Thr62Met): non deleterious LDL receptor missense variants functionally characterized in vitro. Sci Rep. 2018-11-09.
Species/Reactant: Cricetulus griseus (Chinese hamster)
Applications: Western Blotting
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
View moreSpecies/Reactant: Cricetulus griseus (Chinese hamster)
Applications: Western Blotting
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
Galicia-Garcia, U., Benito-Vicente, A., et al. Mutation type classification and pathogenicity assignment of sixteen missense variants located in the EGF-precursor homology domain of the LDLR. Sci Rep. 2020-02-03.
Species/Reactant: Cricetulus griseus (Chinese hamster)
Applications: Western Blotting
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
View moreSpecies/Reactant: Cricetulus griseus (Chinese hamster)
Applications: Western Blotting
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
Galicia-Garcia, U., Benito-Vicente, A., et al. Mutation type classification and pathogenicity assignment of sixteen missense variants located in the EGF-precursor homology domain of the LDLR. Sci Rep. 2020-02-03.
Species/Reactant: Cricetulus griseus (Chinese hamster)
Applications: Western Blotting
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
View moreSpecies/Reactant: Cricetulus griseus (Chinese hamster)
Applications: Western Blotting
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
Galicia-Garcia, U., Benito-Vicente, A., et al. Mutation type classification and pathogenicity assignment of sixteen missense variants located in the EGF-precursor homology domain of the LDLR. Sci Rep. 2020-02-03.
Species/Reactant: Cricetulus griseus (Chinese hamster)
Applications: Western Blotting
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
View moreSpecies/Reactant: Cricetulus griseus (Chinese hamster)
Applications: Western Blotting
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
Galicia-Garcia, U., Benito-Vicente, A., et al. Mutation type classification and pathogenicity assignment of sixteen missense variants located in the EGF-precursor homology domain of the LDLR. Sci Rep. 2020-02-03.
Species/Reactant: Cricetulus griseus (Chinese hamster)
Applications: Western Blotting
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
View moreSpecies/Reactant: Cricetulus griseus (Chinese hamster)
Applications: Western Blotting
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
Key Features
- Purified serum
- Rabbit polyclonal
- Suitable for IHC, Receptor Binding Assays and WB
- Reacts with bovine, human, mouse and rat
Product description
| Quantity | 250 µl (100 µg/ml) |
|---|---|
| Antibody Type | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Application | IHC, Receptor binding assay, WB |
| Purification | Affinity chromatography |
| Reactivity | Bovine, Human, Mouse, Rat |
| Storage | Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
| Intended use | Research use only |
| Immunogen | Specific synthetic peptide (sequence not conserved in VLDL receptor and LRP) of the human LDL receptor extracellular domain (epitope between residues nos. 184-195, the linker region between repeats 4 and 5) |
| Concentration | 100 µg/ml |
| Formulation | PBS with 0.5% BSA |
| Note | Centrifuge prior to opening |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunohistochemistry (IHC) - frozen | 1:20-1:50 |
| Immunohistochemistry (IHC) - paraffin | 1:20-1:50 (microwave treatment recommended) |
| Receptor Binding Assays | Assay dependent |
| Western Blot (WB) | 1:100-1:200 |
Background
The LDL receptor (160 kDa mature or glycosylated receptor; 120 kDa precursor or unglycosylated receptor) plays a key role in cellular cholesterol homeostasis.
The antibody to LDLR reacts specifically with human LDLR and is suitable for use in flow cytometry and immunoblotting. The antibody does not inhibit binding of LDL.
In immunoblotting, the antibody recognizes the 160 kDa band of LDLR and a 120 kDa band of the LDLR precursor from fibroblasts, hepatocytes, and monocytic cells cultured in the presence of lipoprotein-deficient serum.
References/Publications (5)
Publication
Species
Application
Species
hamster
Application
WB
Species
hamster
Application
WB
Species
human
Application
WB
Species
human
Application
WB,IP
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FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
We do not recommend milk for blocking, since the antigen was also found in milk therefore .
Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
The expiration date of our antibodies is indicated on the product label.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet.
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.
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