Epitope mapped
Disease related

anti-Lamin B mouse monoclonal, X223, lyophilized, purified

Cat. No. 61047C

Available, delivery time 1-7 days


Key Features
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with bovine, human, mouse, rat, rat kangaroo and trout
  • Isotype: IgG1
Product description
Quantity 50 µg
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application ICC/IF, IHC, WB
Purification Affinity chromatography
Reactivity Bovine, Human, Mouse, Rat, Rat kangaroo, Trout
Storage before reconstitution 2-8°C until indicated expiry date
Storage after reconstitution Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone X223
Immunogen Nuclear pore complex-lamina fraction of Xenopus laevis (XLKE-A6 cells)
Concentration 50 µg/ml after reconstitution with 1 ml dist. water
Formulation Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
UniprotID A0A3Q1LNG7 (Bovine), P20700 (Human), P14733 (Mouse), P70615 (Rat)
Synonym Lamin-B1, LMNB1, LMN2, LMNB
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:10
Immunohistochemistry (IHC) - frozen 1:10
Western Blot (WB) Assay dependent

The monoclonal antibody decorates the karyoskeleton, i.e. the intermediate filament equivalent of the nucleus.
The epitope was localized to coil 1B (Schumacher et al. 2006).
Polypeptides reacting: Lamin isotypes of Mr 60-75 kDa.

Schumacher, J., Reichenzeller, M., Kempf, T., Schnoelzer, M. and Herrmann, H. Identification of a novel, highly
variable amino-terminal amino acid sequence element in the nuclear intermediate filament protein lamin B2
from higher vertebrates. FEBS Lett. 580, 6211-6216 (2006).

References/Publications (6)
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The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

The epitope of Lamin antibody, clone X223 is located in the helical domain 1B of Lamin. Therefore the antibody should react with the majority of non-phosphorylated lamin molecules. The majority of phosphorylation sites are localized in the non-helical head and tail domains.
Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Please find the solvent for reconstitution and the amount of liquid for reconstitution on the antibody datasheet. The buffer formulation after reconstitution is also mentioned in the datasheet.
PROGEN offers different formats for mouse monoclonal antibodies.  Different antibodies may not be offered in each format, the following are all possible formats:
  1. Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
  2. Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet. 
  3. Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet. 
  4. Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.
We offer bulk amounts starting from 1 mg for many of our mouse monoclonal or recombinant antibodies. Please contact us for further information or use the “bulk button” on the product page.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
It is possible that the antigen is a very minor component in a sample. In such a case enrichment (like a karyoskeletal preparation of the starting cell culture preparation, DNase treatment) is highly recommended.
  • Wash cells 1x with PBS (37°C).
  • Fix in ice-cold (pre-cooled to -20°C) methanol (10 min), followed by ice-cold acetone (5 min).
  • Block with the serum of the species in which the secondary antibody was raised for 30 min.
  • Incubate with primary antibody overnight at 4°C.
  • Wash 3x with PBS at RT.
  • Incubate 1 h with fluorochrome-conjugated secondary antibody at RT.
  • Wash 2x with PBS.
  • Embed in mounting medium.

PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.
  • 5 µm tissue sections.
  • Fixation 10 min in methanol (-20°C) and 1 min in acetone (-20°C).
  • Air drying.
  • Dip slide shortly in PBS.
  • Air drying of the section.
  • Block with the serum of the species in which the secondary antibody was raised for 30 min at RT.
  • Incubation with 1st antibody 1 h at RT or overnight at 4°C in moist chamber.
  • Wash 3x with PBS.
  • Incubation with appropriate secondary antibody (with fluorescence dye), 30-60 min at RT.
  • Wash 3x with PBS.
  • Immerse shortly into ethanol.
  • Let air dry.
  • Cover with mounting medium for microscopy.

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